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Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice

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Abstract

Objective

To investigate the effects of Heijiangdan Ointment (黑绛丹膏, HJD) on oxidative stress in 60Co γ-ray radiation-induced dermatitis in mice.

Methods

Female Wistar mice with grade 4 radiation dermatitis induced by 60Co γ-rays were randomly divided into four groups (n=12 per group); the HJD-treated, recombinant human epidermal growth factor (rhEGF)-treated, Trolox-treated, and untreated groups, along with a negative control group. On the 11th and 21st days after treatment, 6 mice in each group were chosen for evaluation. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), and lactate dehydrogenase (LDH) were detected using spectrophotometric methods. The fibroblast mitochondria were observed by transmission electron microscopy (TEM). The expressions of fibroblast growth factor 2 (FGF-2) and transforming growth factor β1 (TGF-β1) were analyzed by western blot.

Results

Compared with the untreated group, the levels of SOD, MDA and LDH, on the 11th and 21st days after treatment showed significant difference (P<0.05). TEM analysis indicated that fibroblast mitochondria in the untreated group exhibited swelling and the cristae appeared fractured, while in the HJD group, the swelling of mitochondria was limited and the rough endoplasmic reticulum appeared more relaxed. The expressions of FGF-2 and TGF-β1 increased in the untreated group compared with the negative control group (P<0.05). After treatment, the expression of FGF-2, rhEGF and Trolox in the HJD group were significantly increased compared with the untreated group (P<0.05), or compared with the negative control group (P<0.05). The expression of TGF-β1 showed significant difference between untreated and negative control groups (P<0.05). HJD and Trolox increased the level of TGF-β1 and the difference was marked as compared with the untreated and negative control groups (P<0.05).

Conclusion

HJD relieves oxidative stress-induced injury, increases the antioxidant activity, mitigates the fibroblast mitochondrial damage, up-regulates the expression of growth factor, and promotes mitochondrial repair in mice.

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Authors and Affiliations

  1. Department of Oncology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing, 100010, China

    Lin Yang  (杨 霖), Ming-wei Yu  (于明薇), Xiao-min Wang  (王笑民), Guo-wang Yang  (杨国旺) & Xiao-qin Luo  (罗晓琴)

  2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing, 100039, China

    Rui-yun Peng  (彭瑞云), Ya-bing Gao  (高亚兵), Li Zhao  (赵 黎) & Li-feng Wang  (王丽峰)

Authors
  1. Lin Yang  (杨 霖)
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  2. Ming-wei Yu  (于明薇)
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  3. Xiao-min Wang  (王笑民)
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  4. Yi Zhang  (张 怡)
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  5. Guo-wang Yang  (杨国旺)
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  6. Xiao-qin Luo  (罗晓琴)
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  7. Rui-yun Peng  (彭瑞云)
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  8. Ya-bing Gao  (高亚兵)
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  9. Li Zhao  (赵 黎)
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  10. Li-feng Wang  (王丽峰)
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Corresponding author

Correspondence to Xiao-min Wang  (王笑民).

Additional information

Supported by the National Natural Science Foundation of China (No. 30973745)

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Yang, L., Yu, Mw., Wang, Xm. et al. Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice. Chin. J. Integr. Med. 22, 110–115 (2016). https://doi.org/10.1007/s11655-015-2152-z

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  • DOI: https://doi.org/10.1007/s11655-015-2152-z

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