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Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells

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Abstract

Objective

To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction (通莲汤, TLD) on esophageal carcinoma Eca109 cells.

Methods

Eca109 cells were treated with TLD and its separated formulae, including the clearing-heat and detoxification formula (Q), activating-blood and promoting-qi formula (H) and nourishing-yin and blood formula (Z). Cell proliferation was measured using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, cell morphology was observed using a microscope, the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B (NF-κB) signal pathway was detected by Western blot.

Results

The half maximal inhibitory concentrations of TLD, Q and H were 386, 771 and 729 mg/L, respectively. TLD, Q and H significantly inhibited cell proliferation, with 69.43%, 60.84% and 61.90% of treated cells in the G phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD, Q, and H compared with the control group (P<0.05), and TLD showed the strongest effect. Z had no influence on the cell cycle compared with the control group (P>0.05). Western blot detection indicated slight differences in the inhibition of the NF-κB pathway by the different formulae. TLD formula strongly inhibited IKKβ, NF-κB, interleukin-6 and tumor necrosis factor-α expression compared with the control group.

Conclusions

TLD inhibited Eca109 cell proliferation by arresting cells in S phase. The possible mechanism might be related to inhibiting the NF-κB transduction cascade. The combination of the herbs found in the three separate formulae, H, Q and Z, work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation.

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Authors and Affiliations

  1. Basic Section, College of Chinese Medicine, North China University of Science and Technology, Tangshan, Hebei Province, 063000, China

    Yong-sen Jia  (贾永森) & Ji-an Li  (李继安)

  2. Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University, Hangzhou, 310053, China

    Xue-qin Hu  (胡雪琴)

  3. Oncotherm Innovation and Trade Ltd., Pecs, 7322, Hungary

    Szasz Andras

  4. Acupuncture Rehabilitation Foundation, Budapest, 1085, Hungary

    Gabriella Hegyi

  5. Traditional Chinese Medicine Hospital of Shenzhen, Shenzhen, Guangdong Province, 518020, China

    Bing-sheng Han  (韩炳生)

Authors
  1. Yong-sen Jia  (贾永森)
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  2. Xue-qin Hu  (胡雪琴)
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  3. Ji-an Li  (李继安)
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  4. Szasz Andras
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  5. Gabriella Hegyi
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  6. Bing-sheng Han  (韩炳生)
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Corresponding author

Correspondence to Xue-qin Hu  (胡雪琴).

Additional information

Supported by the Natural Science Fundation of Hebei Province of China (No. H2013209053), Scientific and Technological Project of Administration of Traditional Chinese Medicine of Hebei Province, China (No. 2014185)

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Jia, Ys., Hu, Xq., Li, Ja. et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells. Chin. J. Integr. Med. 22, 384–389 (2016). https://doi.org/10.1007/s11655-016-2096-3

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  • DOI: https://doi.org/10.1007/s11655-016-2096-3

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