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Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α

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Abstract

Objective

To study the effect of ligustrazine nanoparticles nano spray (LNNS) on transforming growth factor β (TGF-β)/Smad signal protein of rat peritoneal mesothelial cells (RPMC) induced by tumor necrosis factor α (TNF-α), and the anti-adhesion mechanism of LNNS in the abdominal cavity.

Methods

The primary culture and subculture of rat peritoneal mesothelial cells (RPMC) was processed by trypsin digestion method in vitro. The third generation was identifified for experiment and divided into 5 groups: a blank group: RPMC without treatment; a control group: RPMC stimulated with TNF-α; RPMC treated by a low-dosage LNNS group (2.5 mg/L); RPMC treated by a medium-dosage LNNS group (5 mg/L); and RPMC treated by a high-dosage LNNS group (10 mg/L). Reverse transcription-polymerase chain reaction was applied to test the expression of fifibronectin, collagen I (COL-I), TGF-β mRNA, and Western blot method to test the Smad protein 7 expression of RPMC.

Results

Compared with the blank group, a signifificant elevation in fifibronectin (FN), COL-I and TGF-β mRNA expression of RPMC were observed in the control group (P<0.05). Compared with the control group, LNNS suppressed the expressions of FN, COL-I and TGF-β mRNA in a concentrationdependent manner (P<0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation, and up-regulated with the increase of LNNS dose (P<0.05).

Conclusions

TNF-α may induce changes in RPMC’s viability, leading to peritoneal injury. LNNS could reverse the induction of fifibrosis related cytokine FN, COL-I and TGF-β, up-regulating the expression of Smad7 by TNF-α in RPMC, thus attenuate peritoneal injury by repairing mesothelial cells.

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Authors and Affiliations

  1. Suzhou Hospital of Traditional Chinese Medicine, Suzhou, Jiangsu Province, 215009, China

    Shuai Yan  (颜 帅) & Yin-zi Yue  (乐音子)

  2. Library of Nanjing University of Chinese Medicine, Nanjing, 210023, China

    Lan Yang  (杨 斓), Wen-lin Li  (李文林) & Li Zeng  (曾 莉)

  3. First Clinical Medical College of Nanjing University of Chinese Medicine, Nanjing, 210023, China

    Shuai Yan  (颜 帅) & Li Zeng  (曾 莉)

  4. Yancheng Hospital of Traditional Chinese Medicine, Yancheng, Jiangsu Province, 224000, China

    Jin Yue  (乐 进)

  5. College of Pharmacy of Nanjing University of Chinese Medicine, Nanjing, 210023, China

    Chun-qin Mao  (毛春芹)

Authors
  1. Shuai Yan  (颜 帅)
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  2. Lan Yang  (杨 斓)
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  3. Yin-zi Yue  (乐音子)
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  4. Wen-lin Li  (李文林)
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  5. Li Zeng  (曾 莉)
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  6. Jin Yue  (乐 进)
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  7. Chun-qin Mao  (毛春芹)
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Corresponding author

Correspondence to Li Zeng  (曾 莉).

Additional information

Supported by the National Natural Science Foundation of China (No. 81373843) and College Graduate Student Innovative Research Fund of Jiangsu Province, China (No. CXZZ13-0613) and Administration of Traditional Chinese Medicine Science and Technology Project of Jiangsu Province, China (No. LZ13006)

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Yan, S., Yang, L., Yue, Yz. et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α. Chin. J. Integr. Med. 22, 629–634 (2016). https://doi.org/10.1007/s11655-015-2180-8

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  • DOI: https://doi.org/10.1007/s11655-015-2180-8

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