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Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest

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Abstract

Objective

To investigate whether Shen-Fu Injection (参附注射液, SFI) reduces post-resuscitation immune dysfunction in a porcine model of cardiac arrest by modulating apoptosis of regulatory T lymphocytes (Treg) in the spleen.

Methods

After 8-min untreated ventricular fibrillation and 2-min basic life support, 24 pigs were divided into 3 groups with a random number table, i.e. SFI group, epinephrine (EP) group, and saline (SA) group (8 in each group), which received central venous injection of SFI (1.0 mL/kg), EP (0.02 mg/kg) and SA, respectively. The same procedure without CA initiation was achieved in the sham-operated (sham) group (n=6). After successful return of spontaneous circulation (ROSC), apoptosis rate of splenic Treg was detected by flow cytometry; and the mRNA expression of forkhead/winged helix transcription factor (Foxp3) of splenic Treg was detected by real time-polymerase chain reaction; and the levels of interleukin-4 (IL-4) and interferon-γ (IFN-γ) in porcine splenic Treg were detected by using enzyme-linked immunosorbent assay (ELISA).

Results

Compared with the sham group, the apoptosis rate of Treg was significantly decreased, and the levels of Foxp3 mRNA expression, IFN-γ, IL-4 and IFN-γ/IL-4 were increased in the SA group (P<0.05 or P<0.01). Compared with the EP and SA groups, SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 mRNA expression, IFN-γ and IFN-γ/IL-4 (P<0.05).

Conclusions

SFI has signifificant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen.

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Authors and Affiliations

  1. Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China

    Wei Gu  (顾 伟) & Chun-sheng Li  (李春盛)

  2. Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing, 100020, China

    Wei Gu  (顾 伟), Qian Zhang  (张 茜) & Chun-sheng Li  (李春盛)

Authors
  1. Wei Gu  (顾 伟)
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  2. Qian Zhang  (张 茜)
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  3. Chun-sheng Li  (李春盛)
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Corresponding author

Correspondence to Chun-sheng Li  (李春盛).

Additional information

Supported by the National Natural Science Foundation of China (No. 81372025), Basic and Clinical Research Cooperation Project of Capital Medical University (No. 15JL42), and Beijing Municipal Administration of Hospitals Incubating Program (No. Px2016022)

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Gu, W., Zhang, Q. & Li, Cs. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest. Chin. J. Integr. Med. 22, 666–673 (2016). https://doi.org/10.1007/s11655-016-2505-2

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  • DOI: https://doi.org/10.1007/s11655-016-2505-2

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