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| Study of the effects of the Chinese herbal prescription combined with copper and ferrum on the malignancy of cancer cells |
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| Author Name | Affiliation | | Yong-Chun Chi | Institute of Clinical Medical Sciences, China-japan Friendship Hospital, 100029, Beijing | | Fang Wan | Institute of Clinical Medical Sciences, China-japan Friendship Hospital, 100029, Beijing | | Shu Zhou | Institute of Clinical Medical Sciences, China-japan Friendship Hospital, 100029, Beijing | | Mei Xu | Institute of Clinical Medical Sciences, China-japan Friendship Hospital, 100029, Beijing | | Hao-Ping Du | Institute of Clinical Medical Sciences, China-japan Friendship Hospital, 100029, Beijing |
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| Abstract: |
| As compared with normal cells, cancer cells or malignant cells were morphologically abnormal: their contact inhibition and normal growth order were lost, the DNA content and ploid increased and such kind of cells were transplantable. If the malignancy should be decreased or the malignant cells reversed, the above abnormal changes could be reduced or disappear. BALB/c mice bearing ascites liver cancer were used, Chinese herbal prescription combined with copper and ferrum (CHPCCF) was given by gavage for 10 days, and then some cell-biological parameters were measured; further, the ascites cancer cells (control and treatment) were removed and retransplanted to another mice and observed. The results showed that in CHPCCF treatment group, DNA content of the cancer cells was decreased, and the proliferation index was reduced (control: 83.4 ± 2.6, CHPCCF group: 78.8 ± 1.5; or control: 67.2 ± 1.3, CHPCCF group: 64.2 ± 1.6, P<0.02), the number of the cancer cells in Gl phase increased obviously, but, those of S+G2M phases decreased (P<0.05~0.01); on the DNA histogram, the diploid peak became higher and bigger, but aneuploid or multiploid peaks became smaller. Furthermore, retransplanted experiments showed that in 2/10 animals, the tumors did not grow, and in other 8/10 animals, the tumors grew, but the tumors’ size were smaller than that of the control; the growth inhibition rate was 71. 7% - 88. 3%; and tumors’ grew slowly; the growth curve of the tumors in CHPCCF group was considerably lower than that of the control; the survival period of retransplanted animals was prolonged significantly (from 26.1 ± 11.8 to 38.1 ± 9.6, or to 39.6 ± 7.2 days, P<0.01); the increase in life span was 46% and 52% respectively. The results suggested that CHPCCF could reduce the malignancy of mice liver cancer cells. |
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