中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

Author Name	Affiliation
Xing Chen	Dept. of Cardiology, Tianjin Chest Hospital, 300051, Tianjin
Luo Wang	Department. of Cardiology, First Hospital of Beijing Medical University, 100034, Beijing
Meifu Feng	State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, 100034, Beijing
Guoying Zhu	Department. of Cardiology, First Hospital of Beijing Medical University, 100034, Beijing

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Abstract:

Objective: To observe the effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor (PDGF) in vascular smooth muscle cells (VSMCs) of rats. Methods: The fifth to tenth passage culture of VSMCs was used, tripterine and 20% fetal calf serum added into the medium of cultured VSMCs at concentration of 0. 1 mg/L, 0. 2 mg/L and 0. 3 mg/L after serum-free cultivation for 24 hours.The general RNA was isolated from VSMCs at 6 and 12 hours after the drug addition for detection of oncogene c-myc and PDGF mRNA respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. Results: Tripterine inhibited the expression of PDGF mRNA of VSMCs, and decreased expression of oncogene cmyc mRNA in a dose-dependent manner, either vs. control. Conclusion: Tripterine can inhibit expression of oncogene c-myc and PDGF-A mRNA in VSMCs, therefore it would inhibit overproliferation of VSMCs.