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| Effect of tongbiling on the synoviocyte function in adjuvant arthritis rats |
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| KeyWord:adjuvant arthritis synovial membrane cell culture fibroblast cell proliferation cytokine Tongbiling |
| Author Name | Affiliation | | Jifan Chen | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Huifang Zhao | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Xiaoyan Shen | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Songhua Li | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Yingmin Li | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Shihuang Liao | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Yangmo Huang | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Hideki Okunishi | Shimane Medical University, Japan | | Lianqun Qiu | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Tong Guan | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Yongqi Fang | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou | | Xiaoling Liu | The First Affiliated Hospital of Guangzhou University of TCM, 510405, Guangzhou |
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| Abstract: |
| Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α) and prostaglandin E2(PGE2) secreted by synoviocytes in adjuvant arthritis (AA) rats.Methods: Synovial fibroblast was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL-1, TNF-α bioactivity and PGE2 content of supernatants of cultured synoviocytes were measured.Results: TBL could significantly inhibit the synovial fibroblast proliferation (P < 0.001), and down-regulate IL-1, TNF-α and PGE2 productions (P < 0. 001); indomethacin could obviously promote the synovial fibroblast proliferation (P < 0.001). It significantly inhibited PGE2 production, but further up-regulated IL-1 and TNF-α secreted by synoviocytes (P<0.01).Conclusion: The therapeutical effect of TBL on AA might be associated with its down-regulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels. This program was supported by the Nature Science Foundation of Guangdong Province (No. 960550) |
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