中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

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Effect of dingxin recipe on intracellular free calcium, membrane potential and mitochondria membrane potential in normal and hypoxia myocytes of rat

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Author Name	Affiliation
Yu-hua Jia	Faculty of TCM, The First Military Medical University, 510515, Guangzhou
Xue-gang Sun	Faculty of TCM, The First Military Medical University, 510515, Guangzhou
Xiao-shan Zhao	Faculty of TCM, The First Military Medical University, 510515, Guangzhou

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Abstract:

To elucidate the molecular mechanism on anti-arrhythmic function of Dingxin Recipe (DXR).

Methods

Cardiac myocytes isolated by trypsin method were cultured and labeled with various fluorescence stains. DXR contained serum induced changes of cellular calcium concentration [Ca²⁺]i, cell membrane potential (MP) and mitochondria membrane potential (MMP) were determined with laser confocal microscopy.