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| Study on intervenient effect of angelica sinensis polysaccharide on immunological liver injury and its mechanism in mice |
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| KeyWord:Angelica sinensis polysaccharide, nitric oxide synthase, apoptosis gene, immunological liver injury |
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| Abstract: |
| Objective: To study the changes of expressions of nitric oxide synthase (NOS) of the constitutive type (cNOS) and inducible type (iNOS), the apoptosis related genes bax and bcl-2, as well as the tumor necrosis factor (TNF-α) in immunological liver injury (ILI) and to explore the preventive effects of Angelica sinensis polysaccharide (ASP) on ILI and its mechanism in mice.Methods: ILI model mice induced by intraperitoneal injection of lipo-polysaccharide (LPS) and BCG vaccine were treated with ASP of different doses (30mg/kg, 60mg/kg) by gastrogavage every day for 7 days. The serum alanine transaminase (ALT) and glutathione-S-transferase (GST) activities and NO content in the liver were detected; the expressions of cNOS, iNOS, bcl-2, bax were assessed with immuno-histochemical method, and the TNF-α mRNA expression in the liver was observed by reverse transcriptase-polymerase chain reaction (RT-PCR).Results: Compared with the normal mice , the NO production and ALT, GST levels were raised significantly in the model mice, the TNF-α mRNA expression was also raised significantly. But no obvious changes of cNOS was found. Small dose ASP (30mg/kg) could reduce NO production and ALT, GST levels in model mice by 19.5%, 23.7% and 40.0% respectively, decrease the expression of iNOS and bax by 48.3%, and 26.4%, and increase the expression of cNOS, bcl-2 by 66.9% and 337.3%, respectively, but it could not reduce the TNF-α mRNA expression in the liver. Large dose of ASP (60mg/kg) was not more effective than that of small dose.Conclusion: Changes of NO production and TNF-α mRNA may play an important role in ILI. The mechanism of ASP in intervening ILI may be through modulation on cNOS, iNOS, bax, bcl-2 expression to block the damage of BCG vaccine and LPS on hepatocytes. |
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