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| The potential effect of puerarin in preventing atherosclerosis |
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| KeyWord:Puerarin, antioxidation, active oxygen f ree radical, low density lipoprotein, oxidative modification |
| Author Name | Affiliation | E-mail | | Qing-Lei Zhu | Institute of Geriatric Cardiology, General Hospital of PLA, 100853, Beijing | qlzhu@yahoo.com | | Ai-Xia He | Department of Anesthesiology, the Affiliated Hospital of Weifang Medical College, China | | | Hui-Rong Han | Applied Pharmacology Laboratory of Weifang Medical College, China | | | Xin-Ran Lu | Applied Pharmacology Laboratory of Weifang Medical College, China | | | Shi-Wen Wang | Institute of Geriatric Cardiology, General Hospital of PLA, 100853, Beijing | |
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| Abstract: |
| Objective:To study the activity of Puerarin (Pue) in scavenging oxygen free radical(OFR) and its inhibitory effect on the oxidative modification of low density lipoprotein (LDL). Methods:Riboflavin-light system was used to generate superoxide anion, and Fenton reaction to generate hydroxylfree radical to study the activity of Pue in scavenging OFR. Hydrogen peroxide-induced hemolysis wasused to study the effect of Pue on erythrocyte hemolysis and malondialdehyde (MDA) production. And ul-traviolet ray and cupric sulfate were used to cause the oxidative modification of LDL for studying the inhib-itory effect of Pue on LDL oxidative modification. Results: (1) Pue could, at concentration of 0.01-1.0mmol/L, scavenge superoxide anion radical and at concentration of 7.5-75μmol/L scavenge hydroxyl rad-ical in a concentration dependent manner. (2) Pue could, at concentration of 0.1-10 mmol/L, inhibit sig-nificantly oxidative hemolysis and MDA production of erythrocyte induced by hydrogen peroxide. (3) Pueof 0.01-1.0 mmol/L could inhibit the oxidative modification of LDL in a concentration dependent man-ner. Conclusion: Pue has an anti-peroxidation effect and shows a potential effect in preventing atherosclero-sis. |
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