中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

Author Name	Affiliation
Qing-hua Dong	Cancer Institute, Zhejiang University, 310009, Hangzhou
Shu Zheng	Cancer Institute, Zhejiang University, 310009, Hangzhou
Qing-hua Lu	Cancer Institute, Zhejiang University, 310009, Hangzhou
Li-ming He	Cancer Institute, Zhejiang University, 310009, Hangzhou

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Abstract:

Objective

To study the effect and mechanism of snake venom ofAgkistrodon Halys Pallas in Zhejiang in inducing human leukemic Jurkat cell apoptosis.

Methods

IC₅₀ and growth curve of Jurkat cells were detected by MTT; percentage of apoptotic cells was analyzed by flow cytometry (FCM) using Annexin V FITC/PI staining method; cell cycle was also detected by FCM and protein expression of Bcl-2 gene was detected by FCM and Western-blot.

Results

Zhejiang viper venom could inhibit the growth of Jurkat cells dose-dependently, induce the cell apoptosis, with the bcl-2 protein expression down-regulated. The strongest effect was shown at 48 hrs, it weakened gradually, and the Jurkat cells restored slowly in low concentration of snake venom.

Conclusion

Zhejiang viper venom could induce apoptosis of human leukemic Jurkat cell in dose-dependent manner, the effect is related to the down-regulation of Bcl-2 protein expression.