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| Therapeutic mechanism of Santeng Dingtong recipe on monosodium urate crystal-induced rabbit arthritis |
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| KeyWord:Santeng Dingtong recipe colchicine monosodium urate crystal rabbit arthritis interleukin-1 beta tumor necrosis factor alpha leukotriene B4 enzyme linked immunosorbent assay |
| Author Name | Affiliation | E-mail | | Xie Qiang-min | Key Laboratory of SDA, Medical College of Zhejiang University, 310031, Hangzhou | Xieqm@zju.edu.cn | | Chen Ying | Key Laboratory of SDA, Medical College of Zhejiang University, 310031, Hangzhou | | | Wu Xi-mei | Key Laboratory of SDA, Medical College of Zhejiang University, 310031, Hangzhou | | | Chen Ji-qiang | Key Laboratory of SDA, Medical College of Zhejiang University, 310031, Hangzhou | | | Bian Ru-lian | Key Laboratory of SDA, Medical College of Zhejiang University, 310031, Hangzhou | |
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| Abstract: |
| Objective: To study the therapeutic mechanism of Santeng Dingtong recipe (STDT) on monosodium urate crystal (MSU) induced rabbit arthritis.Methods: Forty-two rabbits were randomly divided into six groups, 7 in each group. Group 1 received 0.9% saline 2.5 ml/kg per day by gastrogavage (ig) for 10 days; Group 2, 3 and 4 received STDT 0.125 g/kg, 1.0 g/kg and 8.0 g/kg per day respectively by ig for 10 days; Group 5 received colchicine 4.5 mg/kg per day by ig for 4 days; and Group 6 was untreated. MSU crystals 10 mg /500 μl containing polymyxin B 10 u/ml was injected into the knee joints of Group 1–5 to make rabbit arthritis models. Leukocytes in synovial lavage fluids was then counted and differentiated; pathological injury of synovial membranes was observed under HE staining; interleukin-1 beta (IL-lβ), tumor necrosis factor alpha (TNFα) and leukotriene B4 (LTB4) content in synovial lavage fluids were determined by ELISA.Results: MSU caused a rapid leukocyte infiltration and increased production of IL-lβ, TNFα and LTB4 2 hrs after intra-articular injection. STDT inhibited neutrophil infiltration in synovial fluids dose-dependently, protected the synovial membrane against pathological injury and reduced the production of IL-lβ, TNFα and LTB4; while colchicine did not decrease the level of TNFα, but significantly inhibited neutrophil infiltration in synovial fluid and reduced the production of IL-11β and LTB4.Conclusion: STDT exerts an anti-inflammatory effect in rabbit model of acute MSU arthritis, its mechanism being probably due to the decrease of IL-1β, TNFα and LTB4 synthesis. This program was supported by the National New Drugs Foundation (No.98-35-N-13) |
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