中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

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Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase

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KeyWord:Nephritis No. 3 recipe, mesangial cell, nitric oxide, nitric oxide synthase

Author Name	Affiliation
Chen Zhi-qiang	Institute of Integrative Chinese and Western Medicine, Hebei Medical University, 050017, Shijiazhuang
Huang Huai-peng	Nanjing University of TCM, China
Huang Wen-zheng	The Affiliated Hospital of Tianjin College of TCM, China
Zhu Xiao-di	The Affiliated Hospital of Tianjin College of TCM, China
Lin Qing-qi	The Affiliated Hospital of Tianjin College of TCM, China

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Abstract:

Objective:To explore the effect of the Nephritis No. 3 (N-3) recipe on nitric oxide (NO), nitric oxide synthase (NOS) secreted by cultured mesangial cells (MC) and its gene expression of the in-ducible nitric oxide synthase (iNOS). Methods:The drug (nephritis No. 3)-containing serum was prepared with serum pharmacological technique, and then was applied to react on mesangial cells cultured in fetal calf serum (FCS) and cells cultured in FCS plus lipopolysaccharide. To observe the secretion of NO and NOS and the gene expression of iNOS by means of RT-PCR. Results:Under the two kinds of culture conditions, the content of NO and NOS in the groups with drug-containing serum were higher than those without drug-containing serum (P<0. 05, P<0. 01), and the expression of iNOS mRNA was up-regulated too. Conclusion: The N-3 could significantly promote the secretion of NO and NOS and the mRNA expression of iNOS in rats.