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| Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α |
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| KeyWord:Naotaifang extract tumor necrosis factor vascular endothelial cell |
| Author Name | Affiliation | E-mail | | Ge Jin-wen | Laboratory of Vascular Biology, Hunan College of TCM, 410007, Changsha, China | cmgjw@163. net | | Chen Song | Laboratory of Vascular Biology, Hunan College of TCM, 410007, Changsha, China | | | Zhu Hui-bin | Laboratory of Vascular Biology, Hunan College of TCM, 410007, Changsha, China | |
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| Abstract: |
| Objective: To observe the changes of coagulation and fibrinolysis function in human umbilical cord vein endothelial cells (HUVEC) induced by recombinant human tumor necrosis factor-α (rhTNF-α) and the effect of Naotaifang extract (NTE) on it.Methods: Cultured HUVEC is randomly divided into six groups: Control group, NTE control group (only 2 g/L NTE), rhTNF-α group (100 μg/ L rhTNF-α), and low-dosage, middle-dosage and high-dosage NTE group (100 μg/L rhTNF-α and 0. 67 g/L, 2 g/L, 6 g/L NTE). The coagulation activity of frozen-dissolved HUVEC, von Willebrand factor (vWF) content in the conditioned medium and tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAD activity were to be detected after 24 hrs.Results: Compared with the control group, PAI activity were enhanced, vWF release markedly increased in conditioned medium of TNF-α group (P < 0.01) and the frozen-dissolved HUVEC markedly shortens the rabbit plasma prothrombin time, and the above changes could be significantly inhibited by the 3 dosages of NTE (P < 0.05,P < 0. 01).Conclusion: NTE is effective in inhibiting the coagulation activity of the HUVEC non-stimulated or stimulated by rhTNF-α to enhance the vWF release, and to adjust fibrinolytic function, and mainly to inhibit the PAI activity. The item is supported by Natural Science Foundation of Hunan Province (No. 98JJY 2027) and Young-middle Aged Science and Technological Fund of Hunan Province (NO. 00JZYZ145) |
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