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| Effect of Buzhong Yiqi decoction on murine liver damage induced by food allergy |
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| KeyWord:food allergy liver damage Buzhong Yiqi decoction |
| Author Name | Affiliation | E-mail | | Chen Hong | Department of Pediatrics, The Third Hospital Affiliated to Sun Yat-Sen University of Medical Sciences, 510630, Guangzhou | chenchenhong@yahoo.com | | Dong Yang-shen | Department of Pediatrics, The Third Hospital Affiliated to Sun Yat-Sen University of Medical Sciences, 510630, Guangzhou | | | Chen Fen-hua | Department of Pediatrics, The Third Hospital Affiliated to Sun Yat-Sen University of Medical Sciences, 510630, Guangzhou | | | Ji Jing-zhi | Department of Pediatrics, The Third Hospital Affiliated to Sun Yat-Sen University of Medical Sciences, 510630, Guangzhou | | | Chen Yan-feng | Department of Pediatrics, The Third Hospital Affiliated to Sun Yat-Sen University of Medical Sciences, 510630, Guangzhou | | | Y. Yikura | Department of Pediatrics, Showa University School of Medicine, 142-0064, Tokyo, Japan | | | Kozo Ueno | Department of Pediatrics, Showa University School of Medicine, 142-0064, Tokyo, Japan | |
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| Abstract: |
| Objective: To investigate the effect of Buzhong Yiqi decoction, BZYQD) on liver damage induced by food allergy in mice.Methods: Nc/Jic strain mice with high levels of serum IgE were sensitized by ovalbumin (OVA), and then divided into two groups and respectively treated with BZYQD (treated group) or normal saline (model group). Samples of serum, liver tissues and small intestine were collected two weeks later, and another group of non-sensitized mice was set as the normal group. The levels of serum alanine aminotransferase (ALT) were measured with spectrophotometry. The liver tissue and small intestine were stained with hematoxylin and eosin (HE) for pathologic analysis. The liver samples were also subjected to analysis of CD4-T helper cell and cytokine (interleukin-4, IL-4, interleukin-6, IL-6) expression with immunohistochemical (avidin-biotin complex, ABC) method.Results: Serum ALT levels decreased and obvious pathologic improvements were seen in the mice treated with BZYQD. And compared with the model mice, the number of positive cells of IL-4, IL-6 and CD4 cell decreased significantly in those treated with BZYQD.Conclusion: BZYQD can effectively decrease the production of cytokines associated with allergic reaction in the liver of mice thus effective in treating liver damage caused by food allergy. |
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