中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

Author Name	Affiliation	E-mail
Qian Li-qi	Nanjing Medical University, China	qianliqi0519@sina.com
Pei Xiao-hua	Pharmaceutical Co. Ltd., Nanjing University of Traditional Chinese Medicine, China
Chen Rong-ming	Jiangsu Provincial Hospital of Chinese Medicine, China
Xu Zhi-yin

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Abstract:

Objective: To investigate the effects of Lirukang oral liquid (LRK) on release of neurotransmitter in rats with hyperplasia of mammary glands (HMG) and to explore its mechanism.Methods: Sixty rats were divided into six groups, the normal control group, the model control group, the large dosage (3.6g/kg) and the small dosage (1.8g/kg) LRK groups, the Ruzengning (RZN, 2.5g/kg) group and the tamoxifen (TAM, 5mg/kg) group, 10 in each group. Except those in the normal control group, all the animals were made into rat model of HMG by intraperitoneal injection of estradiol benzoate. Levels of dopamine (DA) and 5-hydroxytryptamine (5-HT) in hypothalamus and mammary gland in rats were detected by fluorescence luminosity assay, and level of prolactin (PRL) in serum was detected by radioimmunoassay.Results: In the model group, the level of DA reduced significantly (P<0.01), and 5-HT and PRL increased obviously (P<0.01). Compared with the model group, the LRK groups of both dosages and the TAM group had their level of DA significantly increased (P<0.01), and level of 5-HT significantly decreased (P<0.01). The serum PRL in both LRK groups was significantly decreased (P<0.01). No obvious changes in DA, 5-HT and PRL were found in the RZN group.Conclusion: LRK and TAM have similar effects in regulating the release of neurotransmitter in hypothalamus and mammary gland and serum content of estrogen in the animal models of HMG. This study was supported by Jiangsu Provincial Administration Bureau of Traditional Chinese Medicine [No. SZ 95-B18]