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| Effect of propyl gallate on activity of cyclooxygenase 1 and 2 in mice??s peritoneal macrophages |
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| KeyWord:propyl gallate cyclooxygenase 1 cyclooxygenase 2 inflammation |
| Author Name | Affiliation | | Yin Hui-jun | Xiyuan Hospital, China Academy of Traditional Chinese Medcine, 100091, Beijing, China | | Jiang Yue-rong | Xiyuan Hospital, China Academy of Traditional Chinese Medcine, 100091, Beijing, China | | Wu Xiao-hua | Fujian Mindong Rejuvenation Pharmaceutical Co., Ltd., Fuzhou | | Chen Xiao-hong | Institute of Materia Medica, Chinese Academy of Medical Sciences, China | | Chen Ke-ji | Xiyuan Hospital, China Academy of Traditional Chinese Medcine, 100091, Beijing, China |
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| Abstract: |
| Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages.Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F1α(6-keto-PGF1α) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term.Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF1α synthesis at the concentrations of 1 × 10-6, 5 × 10-6 mol/L (P>0.05), but enhanced 6-keto-PGF1α synthesis at the concentrations of 1 × 10-6, 5 × 10-7, 1 × 10-7 mol/L (P<0.01) in vitro, and showed a good dose-dependent manner. It inhibited LPS-induced, COX-2-derived PGE2 synthesis at the concentrations of 1 × 10-6, 1 × 10-6 mol/L (P< 0.05).Conclusion: Within the range of 1 × 10-5 to 1 × 10-7 mol/L, PrG activated COX-1 at lower concentrations and inhibited COX-2 at higher concentrations in murine peritoneal macrophages. This work is supported by the National Natural Science Foundation (item number: 90209054) |
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