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The more efficacious acupoints of Zusanli and Sanyinjiao than that of non-acupoints on bone mass in osteopenic Ovariectomized rats
  
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KeyWord:acupuncture, osteopenia, ovariectomy, bone mineral density, histomorphometry
Author NameAffiliationE-mail
Zhang Wen-ping Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Mie, Japan zhang_wenping@hotmail.com 
Kanehara Masayuki Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Mie, Japan  
Zhang Yan-jun Tianjin College of Traditional Chinese Medicine, Tianjin, China  
Yu Zhi-feng Tianjin College of Traditional Chinese Medicine, Tianjin, China  
Zhang Guo-xia Tianjin College of Traditional Chinese Medicine, Tianjin, China  
Yang You-xin Tianjin College of Traditional Chinese Medicine, Tianjin, China  
Sun Yu-min Tianjin College of Traditional Chinese Medicine, Tianjin, China  
Zhang Jian-mei Tianjin College of Traditional Chinese Medicine, Tianjin, China  
Ishida Torao Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Mie, Japan  
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Abstract:
      Objective: To clarify whether the acupoints of Zusanli (ST36) and Sanyinjiao (SP6) have specific actions other than non-acupoints to bone. Methods: Forty Sprague-Dawley female rats were divided into five groups: Sham operated (sham) group; Ovariectomized (OVX, model) group; non-acupuncture group; OVX, needling on Zusanli and Sanyinjiao (Acp-A) group; OVX, needling on the reverse sides of Zusanli and Sanyinjiao (Acp-B) group; OVX, periostineal stimulation on the same height as points of Zusanli and Sanyinjiao (Acp-C) group. The experiment was continued for 23 weeks and then all animals were sacrificied. Results: OVX had a significantly higher body weight and lower bone mineral density (BMD) on the lumbar vertebrae, total femora and tibiae than sham rats, however, Acp-A showed a higher BMD compared with the other OVX groups. On the other hand, bone weights, bone strength and bone morphometry such as trabecular volume, trabecular separation, labeled width and bone formation rate also showed the same improvements in Acp-A as compared to the other OVX rats. Conclusion: The stimulation on Zusanli and Sanyinjiao specifically prevented the development of osteopenic rats compared with non-acupoints.
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