|
| Effects of Astragalus membranaceus in promoting T-helper cell type 1 polarization and interferon-γ production by up-regulating T-bet expression in patients with asthma |
| |
| View Full Text View/Add Comment Download reader |
|
| KeyWord:Key Word asthma Astragalus membranaceus transcription factor T-box expressed in T cells T helper cells Tinterferon-γ |
| Author Name | Affiliation | E-mail | | Gang Wang | Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China | | | Prof. Chun-tao Liu | Department of Respiratory Disease, West China Hospital, Sichuan University, China | liuchuntao@163.net | | Zeng-li Wang | Department of Respiratory Disease, West China Hospital, Sichuan University, China | | | Cun-liang Yan | Department of Laboratory Medicine, Shenzhen Hospital Affiliated to Peking University, China | | | Feng-ming Luo | Department of Respiratory Disease, West China Hospital, Sichuan University, China | | | Lei Wang | Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China | | | Ting-qian Li | Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China | |
|
| Hits: 1050 |
| Download times: 479 |
| Abstract: |
| OBJECTIVE: To explore the effect of Astragalus membranaceus (AM) on T-helper cell type 1 (Thl) specific transcription factor T-box expressed in T cells (T-bet) expression and Thl/Th2 equilibrium. METHODS: The levels of T-bet mRNA in peripheral blood mononuclear cells (PBMCs) from 15 patients with asthma and 15 healthy subjects were determined by reverse transcription-polymerase chain reaction (RT-PCR). PBMCs in asthma patients were incubated with AM and then the concentration of interferon gamma (IFN-gamma) and interleukin-4 (IL-4) in the supernate before and after AM intervention were determined by ELISA. The numbers of CD4 + CCR3 + and CD4 + CCR5 + cells were counted by flow cytometry. RESULTS: The expression of T-bet mRNA and the level of IFN-gamma were lower, but level of serum IL-4 was higher in asthma patients when compared with those in healthy subjects respectively. After AM (60 microg/ml) intervention, the former two parameters raised and showed a positive correlation between them, while the level of IL-4 was decreased. The mean percentage of CD4 + CCR3 + cells in asthma patients was significantly higher but that of CD4 + CCR5 + cells was lower when compared with those in healthy subjects respectively. After AM intervention, the abnormal change in the two indexes was improved to certain extent, showing a reversing status of Th2 polarization. CONCLUSION: AM could increase the expression of T-bet mRNA and Thl cytokines such as IFN-Y, and might reverse the Th2 predominant status in asthma patients. |
| Close |
|
|
|
