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| Intervention effects of Jianpi Liqi Huoxue Decoction (健脾理气活血汤) on lipid peroxidative liver injury induced by alcohol |
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| KeyWord:Jianpi Liqi Huoxue Decoction, alcohol, liver injury, lipid peroxidation, Lieber-DeCarli model |
| Author Name | Affiliation | E-mail | | Jian-wei Cui | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | | | Prof. Yi-yang Hu | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | yyhuliver@163.com | | Zhi-hong Fang | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | | | Xiao-ning Wang | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | | | Yang Cheng | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | | | Jing-hua Peng | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | | | Qin Feng | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | | | Hui Zhang | Institute of Liver Diseases, Shuguang Hospital, Shanghai University of TCM, Shanghai, 201203, China | |
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| Abstract: |
| Objective To study the intervention effects of Jianpi Liqi Huoxue Decoction (健脾理气活血汤, JLHD) on lipid peroxidative liver injury induced by alcohol. Methods The rat alcoholic model of liver disease (ALD) induced by Lieber DeCarli liquid diet was established. Thirty two male SD rats were randomly divided into 4 groups: the normal group (n=5), the control group (n=9), the model group (n=9) and the JLHD group (n=9). From the 4th week after modeling, the rats were given JLHD or distilled water by gastrogavage respectively, and the samples of blood and liver tissues were taken out from the rats for determination by the end of the 8th week. The hepatic pathological changes were observed with HE staining; the liver injury related indices, including activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, γ-glutamyl transpeptidase (γ-GT) activity and triglyceride (TG) content in liver tissues, as well as the lipid peroxidation related indices, including malonaldehyde (MDA) content and nitric oxide synthase (NOS) activity in liver tissue, serum Fe2+ level, and the anti-peroxidation capacity related indices, including superoxide dismutase (SOD) activity, glutathion (GSH) content and reactive oxygen species (anti ROS) activity in liver tissues were determined. Results (1) There were obvious figures of fatty degeneration and inflammatory infiltration in liver tissues of the model group. As compared with the control group, in the model group, the activity of ALT and AST, and Fe2+ content in serum, γ-GT and NOS activity, TG and MDA content in liver tissues were significantly higher (P<0.01), while the activity of SOD, GSH and anti ROS in liver tissues were significantly lower (P<0.01). (2) The fatty degeneration and inflammatory infiltration of liver tissues in the JLHD group were significantly lessen as compared with those in the model group; and the abnormalities of all the indexes revealed in the model rats were restored to certain extent in the JLHD group, and especially significant were the levels of ALT activity, MDA content and Fe2+, which were nearly normal. Conclusion JLHD has significant effects against alcoholic liver injury, the acting mechanism of which is likely to be related with its anti lipid peroxidative effect. Supported by the National Natural Science Foundation of China (No.30371818) and Project of Key Subject Construction of Shanghai (Y0302) |
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