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| Effect of serum from overfatigue rats on JNK/c-Jun/HO-1 pathway in human umbilical vein endothelial cells and the intervening effect of Tongxinluo (通心络) superfine powder |
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| KeyWord:overfatigue serum vascular endothelial dysfunction signal conduction Tongxinluo superfine powder |
| Author Name | Affiliation | E-mail | | Jun-qing Liang | Department of Pharmacology, Hebei Yiling Pharmaceutical Research Institute, Shijiazhuang, 050035, China | | | Hai-bo Xu | Synthesized Department of the Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, 067000, China | | | Yi-ling Wu | Department of Pharmacology, Hebei Yiling Pharmaceutical Research Institute, Shijiazhuang, 050035, China | jiatcm@163.com | | Shi-ran Sun | Department of Gastroenterology, Traditional Chinese Medicine Hospital of Hebei, Shijiazhuang, 050017, China | | | Zhen-hua Jia | Department of Pharmacology, Hebei Yiling Pharmaceutical Research Institute, Shijiazhuang, 050035, China | | | Cong Wei | Department of Pharmacology, Hebei Yiling Pharmaceutical Research Institute, Shijiazhuang, 050035, China | | | Jia-hua You | Department of Neurology, Traditional Chinese Medicine Hospital of Shijiazhuang, Shijiazhuang, 050017, China | |
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| Abstract: |
| Objective To cultivate human umbilical vein endothelial cells (HUVECs) in the serum of overfatigue rats with the intervention of Tongxinluo (通心络) superfine powder (TXLSP). By examining the variation of the activity of JNK/c-Jun/HO-1 pathway, the possible mechanisms of vascular endothelial dysfunction under overfatigue conditions and the intervening effect of TXLSP were explored. Methods The HUVECs were randomly divided into the normal control group, the model group, the SP600125 (a specifific antagonist of JNK) group, the TXLSP group and the TXLSP + SP600125 group. The content of carboyhemoglobin (COHb) and the leak rate of lactic dehydrogenase (LDH) in different groups were measured. The mRNA and protein expression of JNK, c-Jun, HO-1 and the phosphorylation level of c-Jun (P-c-Jun) were detected using Western blot and PCR methods. Results Compared with the normal control group, the COHb level in supernatant was increased signifificantly in the model group, and the expression of HO-1, JNK, c-Jun mRNA and corresponding proteins and P-c-Jun were also increased remarkably. The increases in these parameters were signifificantly decreased by SP600125. TXLSP showed remarkable up-regulation on the expression of JNK, c-Jun, P-c-Jun and HO-1 mRNA and their protein expression. Compared with the SP600125 group, the expressions of JNK, c-Jun, P-c-Jun and HO-1 mRNA and its protein in the TXLSP+SP600125 group were signifificantly increased at different time points (P<0.05, P<0.01). Conclusions The vascular endothelial dysfunction under overfatigue conditions is related to the activity of the JNK/c-Jun/HO-1 pathway. One of the mechanisms of TXLSP in improving the vascular endothelial function is to adjust the activity of the JNK/c-Jun/HO-1 pathway at gene and protein levels. Supported by the National Key Basic Research and Development Project (973 Project, No. 2005CB523301), the International Science and Technology Cooperation Program (No. 2006DFB32460) |
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