|
| Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model |
| |
| View Full Text View/Add Comment Download reader |
|
| KeyWord:goiter Chinese medicine cell proliferation proliferating cell nuclear antigen cyclin D1 |
| Author Name | Affiliation | E-mail | | Yong Han | Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China | | | Jing Zhou | Department of Endocrinology of Zaozhuang City Hospital, Shandong, 277102, China | | | Shu-jing Yu | Department of Endocrinology of Zaozhuang City Hospital, Shandong, 277102, China | | | Bin Cui | Scientific Centre, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China | | | Hai-qing Zhang | Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China | | | Ling Gao | Scientific Centre, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China | | | Jia-jun Zhao | Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China | jjzhao@medmail.com.cn |
|
| Hits: 1062 |
| Download times: 579 |
| Abstract: |
| Objective To investigate the inhibitory effects of Kangjia Pill (抗甲丸, KJP) on the cell proliferation in rat goiter model induced by methimazole (MMI). Methods Fifty-six Wistar rats were randomly divided into four groups: the normal group, MMI model group (MMI), low dose of KJP group (LKJP), and high dose of KJP (HKJP). Except the normal group (20 rats), the other groups (12 rats in each) were given 0.04% (w/v) MMI through the drinking water until the end of the experiment. One week later, the rats in the LKJP and HKJP groups were given KJP by gastrogavage at the dose of 250 mg/(kg · d) and 1 000 mg/(kg · d), respectively for 12 weeks. The relative thyroid weight (mg/100 g body weight) of each rat was accessed. The expression of proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry, and the correlation analysis between the PCNA positive thyrocytes and the relative thyroid weight was performed. The expressions of PCNA and cyclin D1 were examined with Western blotting. Results After KJP treatment for 12 weeks, compared with the MMI group, the relative thyroid weight of the HKJP group decreased significantly, and the positive thyrocyte populations of PCNA in the two KJP groups reduced markedly (all P<0.05). The correlation analysis showed that PCNA was closely correlated with thyrocyte proliferation (r=0.685, P<0.05). KJP significantly decreased the protein expression of PCNA and cyclin D1 in the thyroid specimens (P<0.05), the high dose showed better effects. Conclusion KJP played a therapeutic role via inhibiting cell proliferation in the rat goitrous glands. Supported by the National Natural Science Foundation of China (No. 30672748), the Natural Science Foundation of Shandong Province (No. 22003C02), and the Shandong Administration of Traditional Chinese Medicine (No. 2005-070) |
| Close |
|
|
|
