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| Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia |
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| KeyWord:Kangquan Recipe benign prostatic hyperplasia testosterone estradiol cell proliferation |
| Author Name | Affiliation | E-mail | | Yuan-peng Huang | Department of Traditional Chinese Medicine, Zhongshan Hospital, Xiamen University, Fujian, 361004, China
Academy of Integrative Medicine, Fujian College of Traditional Chinese Medicine, Fuzhou, 350108, China | | | Jian Du | Academy of Integrative Medicine, Fujian College of Traditional Chinese Medicine, Fuzhou, 350108, China | | | Zhen-feng Hong | Academy of Integrative Medicine, Fujian College of Traditional Chinese Medicine, Fuzhou, 350108, China | zfhong1953@163.com | | Zhi-qing Chen | Department of Traditional Chinese Medicine, Zhongshan Hospital, Xiamen University, Fujian, 361004, China | | | Jin-fa Wu | Department of Traditional Chinese Medicine, Zhongshan Hospital, Xiamen University, Fujian, 361004, China | | | Jin-yan Zhao | Academy of Integrative Medicine, Fujian College of Traditional Chinese Medicine, Fuzhou, 350108, China | |
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| Abstract: |
| Objective To investigate the effects of Kangquan Recipe (康泉方, KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats. Methods Seventy-two SD rats were randomly divided into six groups: the normal group, the model group, the finasteride group, and the low-, middle-, and high-dose KQR groups, 12 in each group. Except those in the normal group, the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with normal saline, finasteride, and low-, middle-, and high-dose of KQR for 30 days. The levels of plasma testosterone (T) and estradiol (E2) were determined by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression ) of proliferating cell nuclear antigen (PCNA) in prostate tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR) after administration. Results Compared with the model group, the prostate weight, the plasma T, and the mRNA expression of PCNA were significantly lower, and the plasma E2 and the ratio of E2/T were higher in the three KQR groups (P<0.05 or P<0.01). There was no significant difference in the prostate weight, plasma T and E2, and ratio of E2/T among the finasteride group and the three KQR groups (P>0.05). The mRNA expressions of PCNA were significantly higher in the middle- and low-dose of KQR groups than those in the finasteride group (P<0.05). Conclusion KQR shows multitarget effects on experimental BPH rats, and the mechanism might be related with regulating the balance of plasma T and E2 and decreasing the PCNAmRNA expression in prostate tissue to restrain cell proliferation in a dose-dependent manner. Supported by the Foundation of Traditional Chinese Medicine of Fujian Province (No. wzy0616), the Foundation of Key Projects from Science and Technology Department of Fujian Province (No. 2008Y0049), and the Foundation of Science and Technology Department of Xiamen (No. 3502Z20084014) |
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