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| Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells |
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| Author Name | Affiliation | E-mail | | Chun-yan Feng | Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China
Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China | | | Xiu-rong Huang | Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China | qihuang@netease.com | | Ming-xin Qi | Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China | | | Song-wen Tang | Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China | | | Yan-hong Hu | Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China | | | Sheng Chen | Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China | | | Fa-jie Ke | Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China | |
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| Abstract: |
Objective To investigate the protective effects of the natural medicinal monomer isopsoralen (ISR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide (H2O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. Methods HLE-B3 cells were treated with H2O2 (300 μ mol/L), β-estradiol (E2: 10?8 mol/L) and H2O2, ISR (10?5 mol/L) and H2O2, or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surfaceenhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (m/z) ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. Results H2O2 up-regulated the expressions of two protein spots (with m/z of 6532 and 6809). E2 mitigated the oxidative damage, and the expression of one protein spot (m/z 6532) was down-regulated. In contrast, ISR down-regulated both of protein spots (m/z 6532 and 6809). Conclusions ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2. |
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