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| Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats |
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| Author Name | Affiliation | E-mail | | Ling Yang | Department of Endocrinology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | | | Kun Lu | Department of Endocrinology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | | | Xiu-ying Wen | Department of Traditional Chinese Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | wenshowing@yahoo.com.cn | | Hao Liu | Department of Traditional Chinese Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | | | Ai-ping Chen | Department of Traditional Chinese Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | | | Ming-wang Xu | Department of Traditional Chinese Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | | | Hong Zhang | Department of Endocrinology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | | | Jie Yu | Department of Endocrinology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430077, China | |
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| Abstract: |
Objective To investigate the antiobesity effect of Jueming Prescription (决明方, JMP), a Chinese herbal medicine formula, and its influence on mRNA expressions of beta3 adrenergic receptor (beta3-AR) and uncoupling protein-2 (UCP-2) in adipose tissue of diet-induced obese rats. Methods Fifty male Sprague-Dawley rats were randomly divided into the normal control group (n =8) that was on a standard chow diet, and the obese model group (n =42) that was on a diet of high fat chow. Two weeks after the high fat diet, 29 obese rats in the obese model group were further randomly divided into 3 groups: the untreated obese model group (n =9), the metformin group (n =10, metformin 300 mg kg?1 day?1), and the JMP group (n =10, JMP 4 g kg?1 day?1). After 8-week treatment, body weight, wet weight of visceral fat, and percentage of body fat (PBF) were measured. The levels of fasting blood glucose, serum lipids, and insulin were assessed, and insulin sensitivity index (ISI) was calculated. The adipose tissue section was stained with hematoxylin-Eosin, and the cellular diameter and quantity of adipocytes were evaluated by light microscopy. The mRNA expressions of beta3-AR and UCP-2 from the peri-renal fat tissue were determined by real-time reverse transcription polymerase chain reaction (RT-PCR). Results Compared with the obese model group, treatment with JMP resulted in significantly lower body weight, wet weight of visceral fat, PBF, and diameter of adipocytes, and significantly higher level of high-density lipoprotein cholesterol, ISI (all P<0.01), JMP increased the mRNA expressions of beta3-AR and UCP-2 from perirenal fat tissue (P <0.05, P<0.01). Conclusions JMP could reduce body weight and adipocyte size; and the effect was associated with the up-regulation of beta3-AR and UCP-2 expressions in the adipose tissue and improvement of insulin sensitivity. |
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