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| A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells |
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| Author Name | Affiliation | E-mail | | Jun Song | Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China | | | Wen-yuan Chen | Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China | | | Li-ya Wu | Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China | | | Liang-pu Zheng | Fujian Academy of Integrative Medicine, Fuzhou, 350108, China | | | Wei Lin | Fujian Academy of Integrative Medicine, Fuzhou, 350108, China | | | Dong Gao | Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China | gd1026@yahoo.com.cn | | Ted J. Kaptchuk | Harvard Medical School, Boston, MA, 02215, USA | | | Ke-ji Chen | Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China | |
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| Abstract: |
Objective To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction (血府逐瘀汤) on the endothelial cell line ECV304. Methods ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoctioncontaining serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray. Results At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed. Conclusion XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h. |
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