中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

Author Name	Affiliation	E-mail
WU An-guo , HUANG Meng-qiu , LI Sheng-mei
ZENG Bao	1. Research Center of New Drug Development, Guangzhou University of Chinese Medicine, Guangzhou (510006), China 2. Research Institute of Mathematical Engineering of Guangzhou University of Chinese Medicine in Dongguan, Dongguan, Guangdong Province (523808), China	zb_111@tom.com

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Abstract:

Objective: To investigate the absorption and transport mechanism of magnolol in Caco-2 cell model. Methods: A human intestinal epithelial cell model Caco-2 cell in vitro cultured was applied to study the absorption and transport of magnolol, the effects of time, donor concentration, P-gp inhibitor verapamil, pH and temperature on the absorption and transport of magnolol were investigated. The determination of magnolol was performed by high performance liquid chromatography, then the values of apparent permeability coefficient (Papp) and Pratio Basolateral-to-Apical (BL-to-AP)/Apical-to-Basolateral (AP-to-BL) were calculated. Results: In Caco-2 cell model, comparing the amounts of transport of AP-to-BL and BL-to-AP, the latter was larger. At the same donor concentration, either the amounts of transport of AP-to-BL or BL-to-AP increased with increase in donor concentration and incubation time. Verapamil could signi?cantly improve the amounts of transport of AP-to-BL. The transport of AP-to-BL and BL-to-AP depended on temperature, and there was no signi?cant effect of pH on the transport of AP-to-BL. Conclusion: Magnolol could be transported through the intestinal mucosa via a passive diffusion mechanism primarily, coexisting with a carrier-mediated transport, at the same time, the ef?ux mechanism could be involved.