中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

Author Name	Affiliation	E-mail
LI Wan-zhen, TAN Ling-ling, LI Qi-jie
DING Wei-jun	College of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu (610072), China	tcmding@yahoo.com.cn

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Abstract:

Objective: To explore natural herbs to maintain the bactericidal activity of hydrogen peroxide (H2O2). Methods: Eighteen extracts of Chinese herbs were prepared complying with the standard protocol. Each of the solutions was then mixed with 1% H2O2. The mixtures were handled with two approaches: autoclaved daily for one, two or three times; stored at room temperature from one through five years. Then the bactericidal activity were evaluated by assaying the minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) against Gram positive (Staphylococcus aureus, ATCC25923) and Gram negative (Escherichia coli , ATCC12421) bacteria. Results: While mixed with 1% H2O2, 10 out of 18 kinds of assessed Chinese herbs displayed MBC values at 1:12800 or higher after three times of autoclaving, and 8 of them preserved such level of MBC value after stored at room temperature for three years. Some Chinese herbs, i.e. R. Scutellariae, R. Coptidis, R. Bupleuri , H. Epimedii , C. Phelledendri and F. Chrysanthemi , can significantly maintain the bactericidal activity of diluted H2O2. Conclusions: Certain Chinese herbs can effectively stabilize the bactericidal activity of H2O2 undergoing autoclave or long-term storage. This paper reported a brandnew pharmaceutical function of Chinese herbs and provided experimental data for the potential enhancement of H2O2 usage while its stability level is promoted.