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| Effect of Two Chinese Medicinal Compounds, Blood-Activating and Water-Draining Medicine, on Tumor Necrosis Factor α and Nuclear Factor κB Expressions in Rats with Intracerebral Hemorrhage |
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| KeyWord:intracerebral hemorrhage, cerebral edema, tumor necrosis factor alpha, nuclear factor kappaB, blood-activating, water-draining, Chinese medicine |
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| Abstract: |
| Objective: To investigate the effect of blood-activating Chinese medicinal compounds and water-draining Chinese medicinal compounds on tumor necrosis factor alpha (TNF-α) and nuclear factor kappaB (NF-κB) expressions in rats with intracerebral hemorrhage (ICH) at the acute stage, and to monitor their therapeutic effect and mechanism of action on inflammation and cerebral edema. Methods: A rat model of cerebral hemorrhage was achieved by injecting autologous arterial blood into the caudate nucleus. A total of 168 rats were randomly divided into 4 groups: blood-activating medicine group (n=42), water-draining medicine group (n=42), sham operated group (n=42), and the model group (n=42). A series of brain samples were obtained at days 1, 3 and 5 after ICH from rats in all groups. Protein expression levels of TNF-α and NF-κB were measured by immunohistochemical staining and gene expression levels of TNF-α and NF-κB were measured by real-time fluorescent PCR. Results: Compared to the sham operated group, protein expression levels of TNF-α and NF-κB in the model group significantly increased (P<0.01). Protein and gene expressions of TNF-α from the blood-activating medicine group and water-draining medicine group significantly decreased when compared to those in the model group (P<0.05). Meanwhile, compared to the model group, the expression of NF-κB in the blood-activating medicine group significantly decreased (P<0.05), while expression of NF-κB in the water-draining medicine group did not differ (P>0.05). Conclusions: Blood-activating Chinese medicinal compounds and water-draining Chinese medicinal compounds can alleviate inflammation of peripheral tissue and cerebral edema. However, the blood-activating Chinese medicinal compounds were more effective than the water-draining Chinese medicinal compounds. The possible effective mechanism may be by means of inhibiting the activation of NF-κB so as to suppress the transcription of target genes including gene expression of TNF-α. |
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