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| Influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in Rats with Experimental Autoimmune Uveitis |
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| KeyWord:Yanyankang powder, experimental autoimmune uveitis, gamma interferon, interleukin-10, Lewis rat, Chinese medicine |
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| Abstract: |
| Objective: To study the influence of Yanyankang Powder (眼炎康散) on Th1/Th2 in rats with experimental autoimmune uveitis (EAU). Methods: The EAU models were induced in Lewis rats by immunization with interphotoreceptor retinoid binding protein (IRBP) 1177–1191 in complete Freund's adjuvant. The rats were randomly divided into 3 groups: a model control group, a Yanyankang group, and a prednisone group, 9 rats in each group. The model control group was intervened with saline solution by gavage. The Yanyankang group was intervened with Yanyankang Powder 4 g/(kg?day) by gavage. The prednisone group were intervened with prednisone acetate tablets 5 mg/(kg?d) by gavage. All groups were intervened after immunization once every 2 days for 18 days and monitored by slit-lamp biomicroscopy daily until day 18. The levels of gamma interferon (INF-γ) and interleukin-10 (IL-10) in the supernatants of T cells were determined by enzyme-linked immunosorbent assay. Polymerase chain reaction (PCR) technology was used for measuring Th1 and Th2 related cytokine mRNA expressions. Results: Slighter intraocular inflammation was found in the Yanyankang group and the prednisone group than the control group. The levels of the IFN-γand IL-10 in the supernatants of the spleen lymph node cells were 382.33±6.30, 155.87±4.46 μg/L in the Yanyankang group and 270.93±7.76, 265.32±11.88 μg/L in the prednisone group. Both had significant differences compared with the control group (941.53±8.59, 20.67±4.65 μg/L; P=0.01). The PCR results showed the same tendency. Conclusion: Yanyankang Powder showed favorable effects in the rats with EAU by influencing the function of Th1 and Th2 cells. |
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