中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

Author Name	Affiliation	E-mail
YIN Qin, WU Cai-jun, YANG Jun
LI Chun-sheng	Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonarycerebral Resuscitation, Beijing (100020), China	lcscyyy@163.com

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Abstract:

Objective: To investigate the effects of Shenfu Injection (参附注射液, SFI) on cerebral metabolism in a porcine model of cardiac arrest (CA). Methods: Thirty Wuzhishan minipigs were randomly assigned to the control group (n=6), epinephrine group (EP group, n=12) and SFI group (n=12). After 8 min of untreated ventricular fibrillation (VF), pigs in the EP group or SFI group were administered with either EP (0.02 mg/kg) or SFI (1.0 mL/kg), respectively. After successful resuscitation, cerebrospinal fluid (CSF) levels of glucose, pyruvate, lactate, glutamate and glycerol were measured at 1, 6, 12 and 24 h after recover from spontaneous circulation (ROSC). In addition, neurologic deficit score (NDS) was calculated at 24 h after ROSC. Surviving pigs were killed at 24 h after ROSC, and the brain tissue was obtained for ultra-microstructure examination. Results: Compared with the EP group, CSF glucose and pyruvate levels were higher (all P<0.01), and lactate levels were lower in the SFI group (P<0.01). Meanwhile, CSF glutamate and glycerol levels in the SFI group were lower in comparison to the EP group (all P<0.05). In addition, SFI decreased NDS at 24 h after ROSC (P<0.01), and alleviated the histopathological damage of the brain. Conclusions: SFI could alleviate brain injury after CA, which may be associated with improving cerebral metabolism.