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| Shexiang Tongxin Dropping Pill (麝香通心滴丸) Protects against Na2S2O4-Induced Hypoxia-Reoxygenation Injury in H9c2 Cells |
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| KeyWord:myocardial ischemia, apoptosis, Shexiang Tongxin Dropping Pill, Chinese medicine, Na2S2O4-induced hypoxia-reoxygenation injury |
| Author Name | Affiliation | E-mail | | LIN Shan, LIN Jiu-mao, ZHANG Ling | | | | PENG Jun | 1. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
2. Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
3. Rainbow Babies Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH (44106), USA | pjunlab@hotmail.com |
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| Abstract: |
| Objectives: To investigate the protective effects of Shexiang Tongxin Dropping Pill (麝香通心滴丸,STP) on Na2S2O4-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells. Methods: The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following Na2S2O4-induced hypoxia using Hoechst staining, annexin V/propidium iodide (PI) flow cytometry, real-time polymerase chain reaction and Western blot analysis. Results: STP pretreatment significantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by Na2S2O4 treatment (P<0.05). In addition, STP pretreatment attenuated Na2S2O4-induced hypoxic damage, down-regulated the expression of pro-apoptotic Bax, and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells (P<0.05). Conclusions: STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis. These results further support the use of STP as an effective drug for the treatment of ischemic heart disease. |
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