中国结合医学杂志（英文版）Chinese Journal of Integrative Medicine

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Arginine Vasopressin-Aquaporin-2 Pathway-Mediated Dehydration Effects of Electroacupuncture in Guinea Pig Model of AVP-Induced Endolymphatic Hydrops

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KeyWord:endolymphatic hydrops, electroacupuncture, arginine vasopressin (AVP)-aquaporin-2 (AQP2) pathway, Meniere's disease

Author Name	Affiliation	E-mail
JIANG Li-yuan, HE Jiao-jun, CHEN Xi-xi
CHEN Hua-de	Department of Acupuncture and Moxibustion, the Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou (310053), China	docchd@sina.com

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Abstract:

Objective: To investigate the effects of electroacupuncture (EA) on endolymphatic hydrops (EH) and the regulation of arginine vasopressin (AVP)-aquaporin-2 (AQP2) pathway in guinea pigs. Methods: EH was induced in male guinea pigs by an intraperitoneal injection of AVP. For the treatment, EA was delivered to Baihui (GV 20) and Tinggong (SI 19) acupoints, once per day for 10 consecutive days. In histomorphological studies, cochlear hydrops degree was evaluated by hematoxylin-eosin (HE) staining, and then the ratio of scala media (SM) area to SM + scala vestibuli (SV) area (R value) was calculated. In mechanical studies, a comparison of plasma AVP (p-AVP) concentrations, cyclic adenosine monophosphate (cAMP) levels, vasopressin type 2 receptor (V2R) and AQP2 mRNA expressions in the cochlea were compared among groups. Results: EA significantly reduced cochlear hydrops in guinea pigs (P=0.001). EA significantly attenuated the AVP induced up-regulation of p-AVP concentrations (P=0.006), cochlear cAMP levels (P=0.003) and AQP2 mRNA expression (P=0.016), and up-regulated the expression of V2R mRNA (P=0.004) in the cochlea. Conclusion: The dehydrating effect of EA might be associated with its inhibition of AVP-AQP2 pathway activation.