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| Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor |
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| KeyWord:Prunella vulgaris polysaccharide, carcinoma-associated fibroblasts, basic fibroblast growth factor, SKBr-3, Chinese medicine |
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| Abstract: |
| Objective: To study the effects of Prunella vulgaris polysaccharide (PVP) on human breast carcinoma-associated fibroblasts (CAFs). Method: Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)-2H-tetrazolium (MTS) assay. Wound healing experiment and transwell migration assay were used to investigate the anti-migration effects. Flow cytometry was applied to detect cell apoptosis and cell cycle distribution. Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor (bFGF) in CAFs. Culture SKBr-3 with CAFs conditioned medium (CAFs-CM) to evaluate the indirect function on the proliferation of breast cancer SKBr-3 cells. Results: PVP inhibited the viability of CAFs by inducing apoptosis (P<0.01) and arresting cell cycle (P<0.01). It also inhibited the migration of CAFs (P<0.01). bFGF promoted CAFs proliferation (P<0.01) and migration (P<0.01), protected CAFs from apoptosis (P<0.05) and reduced G0 phase to 49.06% (P<0.01). However, these effects of bFGF on CAFs could be abrogated by PVP. Culturing SKBr-3 with CAFs-CM, PVP could inhibit the viability of breast cancer SKBr-3 cells indirectly. Moreover, PVP reduced the mRNA expression (P<0.01) and protein secretion of bFGF (P<0.01) in CAFs. Conclusion: PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expression, thus inhibiting the growth of breast cancer SKBr-3 cells indirectly. |
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