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| Inhibition of Proliferation of Prostate Cancer Cell Line DU-145 in vitro and in vivo Using Salvia miltiorrhiza Bunge. |
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| KeyWord:apoptosis, prostate cancer, Salvia miltiorrhiza Bunge., Chinese medicine |
| Author Name | Affiliation | E-mail | | Woong Jin Bae, Jin Bong Choi, Kang Sup Kim | | | | Sae Woong Kim | 1. Department of Urology, The Catholic University of Korea, College of Medicine, Seoul (06591), Republic of Korea
2. Catholic Integrative Medicine Research Institute, College of Medicine, The Catholic University of Korea, Seoul (06591), Republic of Korea | ksw1227@catholic.ac.kr |
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| Abstract: |
| Objective: To investigate the antiproliferative activity of Salvia miltiorrhiza Bunge. (SM) on the castration-resistant prostate cancer (CRPC) cell line DU-145 in vitro and in vivo. Methods: Prostate cancer cell line (DU-145) and normal prostate cell line (RWPE-1) were treated with SM at different concentrations (3.125, 12.5, 25 and 50 μ g/mL) to investigate the antiproliferative effects. DNA laddering analysis was performed to investigate the apoptosis of DU-145 cells. Molecular mechanism was investigated by Western blot analysis of p53, Bcl-2, prostate specific antigen (PSA), and androgen receptor (AR). Six-week-old male BALB/c nude mice were randomly divided into normal control group (n=101) and treated group (n=101) which administered 500 mg/kg SM for 2 weeks. Tumor volumes were measured. Results: Treatment with SM resulted in a dose-dependent decrease in cell number of DU-145 cells in comparison with RWPE-1. DNA laddering analysis indicated the apoptosis of DU-145 cells. Treatment with SM increased the expression of p53 and reduced the expression of Bcl-2 proteins. The levels of PSA were considerably reduced in SM-treated group compared to the controls, and a decrease in AR expression was observed when cells were treated with SM in the same pattern as a reduction in PSA. In the tumour xenograft study, SM given once a day for 2 weeks significantly inhibited tumour growth. Conclusion: SM might contribute to the anticancer actions such as induction of apoptosis and inhibition of proliferation of prostate cancer cells. |
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