淫羊藿肉苁蓉对羟基脲所致“阳虚”动物脱氧核糖核酸合成率的作用

作者	单位
刘福春	第二军医大学第一附属医院中医科
丁光霞	第二军医大学第一附属医院中医科
李菊仙	第二军医大学第一附属医院中医科

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中文摘要:

本文选用羟基脲制造"阳虚"动物模型,用~3H-TdR 体内掺入 DNA 的方法,研究淫羊藿肉苁蓉对"阳虚"动物 DNA 合成率的作用。作者认为羟基脲因其抑制核苷酸在二磷酸化水平的还原反应而导致 DNA 合成率降低,蛋白质、能量代谢紊乱造成"阳虚";淫羊藿肉苁蓉可能含有一种激活核苷酸还原酶的生物活因子,因而能显著地提高"阳虚"动物DNA 合成率。

英文摘要:

The effects of Yang-invigorating drugs Epimeaium sagittatum and Cistanche deserticola on"Yang- insufficiency"mice were studied with a new"Yang-insufficiency"animal model induced by hydroxyurea as an inhibitor of nucleotide reductase.The animal used were divided into three groups:(1)the normal control group(8 animals),(2)the"Yang-insufficiercy"group(8 animals),(3)the group with"Yang- insufficiency"treated with Epimedium sagittatum and Cistanche deserticola(9 animals).The rates of DNA synthesis in the liver and spleen(cpm/mg of DNA/minute,M±SE)of the groups were found to be 11,560±1,980,5,600±848 and 9,900±1,660 respectively.This rate of the"Yang-insufficiency"group was significantly lowered(P<0.05).It can be seen that Epimedium sagittatum and Cistanche deserticola can raise the rate of DNA synthesis of the"Yang-insufficiency"animals,and thus improve the conditions of the animals. The"Yang-insufficiency"caused by hydroxyurea is believed to be due to inhibition of the activity of nucleotide reductase,which keeps four kinds of nucleotide at the diphosphate level,unable to be reduced to DNA,and thus leads to the reduction of DNA synthesis,bringing on disturbance in metabolism of protein and fat and symptoms of"Yang-insufficiency".Epimedium sagittatum and Cistanche deserticola might contain certain bietical active agent,which could activate nucleotide reductase,counteract the inhibiting hydroxyurea and keep normal metabolism of DNA in the body.

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