| 谢锦玉,屠国瑞,李风文,王玉萍,何伟,刘铭福,王勤明.党参对巨噬细胞作用的细胞化学研究[J].中国中西医结合杂志,1985,(8):487-488,513,453 |
| 党参对巨噬细胞作用的细胞化学研究 |
| Effect of Radix Codonopsis Pilosulae on Cytochemical Changes of Macrophage |
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| DOI： |
| 中文关键词: 腹腔巨噬细胞 化学研究 明党参 化学成份 显微分光光度计 小白鼠 定量测定 显著差异 对照组 单个细胞 |
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| 中文摘要: |
| 应用细胞化学定性、定位和定量技术研究了党参对小白鼠腹腔巨噬细胞的细胞化学成份的变化。实验证明党参能使巨噬细胞的数量明显增加,细胞体积增大,伪足增多,吞噬能力增强。细胞内的 DNA、RNA,糖类,ACP 酶,ATP 酶,酸性酯酶及琥珀酸脱氢酶活性均显著增强。并用显微分光光度计对上述单个细胞的某些化学成份进行定量测定,结果党参组与对照组有显著差异。本研究为扶正固本中药的应用和作用机理提供了一定的实验依据。 |
| 英文摘要: |
| The effect of Radix Codonopsis Pilosulae was studied by means of qualitative and quantitative analysis and localization of cytoehemicals.Results showed that the number of macrophage and its pseudopods were markedly increased,the cell volume enlarged and the action of phagocytosis enhanced.The activities of intracellular DNA, RNA,carbohydrate,acid phosphatase(AcPase),adenosine triphospbatase(ATPase),acid α-naphthyl acetic esterase (ANAE)and succinate dehydrogenase(SDH)were remarkably enhanced,moreover,microspectrophotometer was used for quantitative analysis of some chemical substances of the above mentioned individual macrophage,and the results indicated that there was obvious difference between the Radix Codonopsis Pilosulae group and the control group(P<0.05～0.001),The present study has provided certain experimental evidence for the mechanism and application of the TCM theory of strengthening the body resistance to consolidate the constitution. |
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