阿魏酸钠抗红细胞膜脂质过氧化的作用

作者	单位
潘华珍	北京协和医科大学
冯立明	北京协和医科大学
许彩民	北京协和医科大学
张之南	北京协和医科大学
赵崇义	美国北加州儿童医院医学中心
余潮洁	美国北加州儿童医院医学中心
徐理纳	中国医学科学院药物研究所

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中文摘要:

本文报道在体外用各种方法使正常人及溶血性贫血患者红细胞氧化,同时加入从当归提取的纯品阿魏酸钠,观察其对红细胞脂质过氧化的影响。结果表明阿魏酸钠可减少 H₂O 及·O₂^-引起的脂质过氧化反应;有抗·OH 及 MDA 的溶血作用。对其作用机理进行探讨,发现阿魏酸钠有直接抗 H₂O₂作用;对 MDA 无影响;可与膜磷脂酰乙醇胺结合,可能是阿魏酸钠保护膜脂质不受氧化的原因之一。

英文摘要:

Sodium ferulate（SF）is an active ingredient from a Chinese herb,Angelica sinensis,used for inhibition of platelet aggregation.It was attempted to see if SF could inhibit lipid peroxidation of red blood cells and prevent hemolysis of paroxysmal nocturnal hemoglobinuria（PNH）erythrocytes.Normal and PNH erythrocytes were incubated with melonyl dialdehyde（MDA）and SF,percentage of hemolysis was measured,SF could remarkably reduced hemolysis induced by MDA.Sickle-cell anemia erythrocytes was incubated with zymosan actived neutrophils and SF.The concentration of peroxidation product MDA was measured.It showed that the higher the concentration of SF,the lower the MDA product.SF was incubated with MDA and H₂O₂ separately and concentrations of MDA and H₂O₂ were measured. MDA concentration did not change after SF treatment,where H₂O₂ concentration was significantly reduced.SF could combine with phosphorylethanoamine,which may be the main cause of protection of lipid peroxidation.

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