丹参酮对人白细胞趋化性影响的观察

作者	单位
高骥援	中国医科大学病理生理教研室
王淑芬	中国医科大学病理生理教研室
张克坚	中国医科大学病理生理教研室
王常慧	中国医科大学病理生理教研室

摘要点击次数: 1472

全文下载次数: 950

中文摘要:

本文应用琼脂糖玻片法进一步证实了丹参酮的抗炎效应,并探索了应用体外法白细胞趋化性实验进行抗炎药物筛选的可行性。研究表明,当健康人白细胞(5×10~7/ml)与50ng/ml 丹参酮共同孵育1小时时,可使白细胞趋化性发生明显抑制;而对白细胞的随机运动无影响。若孵育时间持续19小时,则5 ng/ml 丹参酮足以使白细胞趋化性及随机运动均发生有意义的抑制。

英文摘要:

As has been demonstrated by a series of clinical practices and experimental researches,Tanshinone(ether extract from the root of Salvia miltiorrhiza Bunge)possesses antibiotic and antiin flammatory actions.However it has not been reported whether Tanshinone has inhibitory action on leukocyte chemotaxis in vitro.This paper reports the observation ofactions of Tanshinone on leukocyte random locomotion and chemotaxis in vitro by agarose plate assay for elucidation of Tanshinone’s action.The results shows though h uman leukocytes chemotaxis was inhibited when Tanshinone(50 ng/ml)and leukocytes suspensions(10~7/ml)preincubated for 1 hour at 37℃,random locomotion showed no changes.When action of Tanshinone on leukocytes lasted 19 hours,both chemotaxis and random locomotion were inhibited by Tanshinone with the same concentration.The results demonstrate and confirm the antiinflammatory action and mechanism of Tanshinone.

关闭