中药活血化瘀片对于实验性肝损伤的效果研究

The effect of herbal mixture Qucen Tablet（QT）on the liver injury by carbon tetrachloride in the rat was evaluated.Liver injury was induced in female Sprague Dawley rats（initial weight 120～140 g）by a combination of oral phenobarbital（0.5 g/L in the only drinking water provided）and CCI₄ inhalation.Animals received CCI₄ 2～3 times a week for 8 weeks and a closed chamber for periods ranging between 2 and 10 minutes.The animals were divided into 4 groups.Group A:QI only.Group B:CCI₄ plus phenobarbital.Group C:CCI₄ plus phenobarbital plus QT,Group D:normal control.For a measure of demethylation by the P₄₅₀enzyme system,the Kel was measured initially and just before sacrificed.Protein bound hydroxyproline was measured in the livers at sacrifice by the method of Rojkind and Gonzale. The deaths of Group A.B.C and D:0/32,9/32,1/32 and 0/11.The liver weight/body weight:2.89%.4.29%, 3.73% and 2.74%.The hepatic protein bound hydroxyproline:3.47±0.7,9.58±1.5,13.0±1.9 and 5.71±0.7（μmol）. The aminopyrine Kel(×10^-3)intially:10.93±0.28.10.54±0.54,10.53±0.39 and 11.64±0.27;finally:8.25±0.25, 11.49±0.48,11.11±0.40 and 8.23±0.44,Histology% cirrhotic:0,9/28,1/32 and 0. These data suggest that QT is effective in reducing the liver injury by carbon tctrachloride and phenobarbital. There was a significant reduction in mortality and in the ratio of liver weight to body weight.Histologic cirrhosis was diminished in the Group receiving QT compared to those receiving only carbon tetrachloride and phenobarbital. The effect seems to be independent of the P₄₅₀enzyme system as QT did not appear to effect demethylation by this enzyme system in the presence of carbon tetrachloride and phenobarbital.

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