益母草抗血小板聚集的机理研究

作者	单位
张陈福	上海中医学院中医实验研究室
朱晓梅	上海中医学院中医实验研究室
宫斌	上海中医学院同位素室
莫启忠	上海中医学院同位素室
李承珠	上海医科大学病理生理教研室
杨诗春	上海医科大学病理生理教研室

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中文摘要:

本文研究了益母草注射液对小鼠血小板内 cAMP、cGMP 以及大鼠颈动脉壁PGI₂样物质的影响。证明益母草能使小鼠血小板内 cAMP 含量升高,显著抑制血小板聚集,但对大鼠血管壁 PGI₂样活性物质无影响。其作用机制很可能是药物具有抑制磷酸二酯酶活性,或通过激素等起作用。

英文摘要:

Seventeen IcR mice of either sex weighing 22～28 g were used for investigating the effect of motherwort（MW） on cAMP and cGMP concentration of platelets.The animals received 0.05 ml/100 g body weight of MW preparation via tail vein（contains crude drug 2g/ml）.Control animals received same dose of normal saline.Studies on cAMP and cGMP concentrations of platelets were done to PRP,obtained from blood drawn via internal carotid artery thirty minutes after drug administration.An experiment was also designed for detecting the effect of MW on PGI₂ level of vessel wall.0.5 ml 100 g body weight of MW or normal saline were introduced into jugular vein of male Sprague-Dawley rats weighing 250～300 g under 3% sodium pentothal anesthesia.Five minutes later,a fragment of carotid artery 1 mm in length was resected and preserved in ice water after washing.Still 5 minutes later,platelet aggregation test induced by ADP was done to the PRP isolated from aortic blood to which MW or normal saline was then added. Experimental data reveal that MW can cause a significant increase of platelet cAMP concentration（P<0.01 as compared with controls）and also inhibit platelet aggregation but has no influence on the PGI₂ level of the vessel wall.The results suggest that MW may possess a β-adrenergic pharmacologic action that raises the cAMP concentration of platelets through the inhibitory action of phosphodiesterase.（Original article on page 39）

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