轮状病毒肠炎的实验诊断研究及其治疗

作者	单位
叶礼鸣	福建医学院
叶孝礼	福建医学院
白植生	卫生部兰州生物制品研究所
吴妙灵	卫生部兰州生物制品研究所

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中文摘要:

本文对52例婴幼儿秋冬季腹泻及32例正常对照的粪便标本进行轮状病毒病原检测比较研究。腹泻组的52例中单克隆抗体酶联免疫吸附试验(单抗 ELISA)阳性42例(80.8%);聚丙烯酰胺凝胶电泳(PAGE)阳性37例(71.2%);多克隆抗体酶联免疫吸附试验(多抗 ELISA)阳性36例(69.2%);电镜检测38例,阳性24例(63.2%)。在正常对照的32例中:单抗 ELISA 阳性6例(18.8%);PAGE 全部阴性;多抗 ELISA 阳性7例(21.8%)。经比较证实,单抗 ELISA 是临床实验室检测轮状病毒的可靠方法。选用中药止泻定治疗轮状病毒肠炎,治疗组平均止泻天数比对照组缩短,有显著性差异。

英文摘要:

The purpose in the study was to compare the sensitivity of monoclonal antibodies(McAb)ELISA with polyacrylamide gel electrophoresis(PAGE),polyclonal antibodies(PcAb)ELISA,and electron microscopy (EM)for the detection of human rotavirus in stools from infants with acute diarrhea.The samples from 52 diarrheal children and 32 normal children were examined,with the results that the positive rate of diarrheal group and normal control by McAb ELISA were 80.8%(42/52)and 18.8%(6/32),71.2%(37/52)and 0%(0/32)by PAGE,69.2%(36/52)and 21.8%(7/32)by PcAb ELISA respectively.The EM positive rate of diarrheal group was 63.2%(24/38).The results showed that McAb ELISA was a reliable method for detection of rotavirus in a clinical laboratory.It was suggested that Zhi Xie Ding(止泻定)was an effective drug for the treatment of infantile diarrhea caused by rotavirus,the curing period was significantly shortened than the control.

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