抗戾散抗病毒及诱生干扰素作用

本实验观察抗戾散的抗病毒及诱生干扰素作用。结果表明,该方具有明显的直接灭活滤泡性口炎病毒作用;1:100浓度为 L₉₂₉细胞的无毒限量,此浓度作用细胞24小时可有效保护 VSV 的攻击,其病毒抑制滴度为2个对数值。该方具有促进新城鸡瘟病毒诱生小鼠干扰素的作用。干扰素滴度（u/ml）实验组2^9.17±0.32与对照组2^8.01±0.22相比差异显著,P<0.001。本实验为抗戾散治疗病毒性传染病提供了依据。

英文摘要:

This paper presents the anti-viral and interferon-inducing effect of Kangli Powder（KLP）with the model of VSV-L929 cells.KLP was composed of Chinese herbal drugs according to the pestilence theory of TCM.Result:（1）It inactivated VSV directly and the effect of inactivation was strength- ened with the increase of consistency.1:100 consistency was the maximal non-toxic dosage limit. This.consistency that acted on cells for 24 hours was able to protect them effectively from the attack of 100 TCID50 VSVs.The test of inhibiting virus reproduction proved that inhibiting titre was two logarithmic value.（2）KLP promoted NDV inducing the interferon of mice.100%KLP 0.4ml fed through gastric tube in the mice,once a day in the experimental group,this dosage was used for seven days successively.In the seventh day,1280HAU/ml NDV 0.5 ml was injected into the abdominal cavity of mice.The mice were let out the blood till death six hours later,The titre of interferon in serum was examined.The mean titre was 2^8.01±0.22 in the control group and 2^9.17±0.32 （u/ml,（?）±SD）in the experimental group;by comparison between this group and control,the difference was highly significant,P<0.001.（3）KLP induced the interferon directly.100%KLP 0.6 ml Was injected into the abdominal cavity of the experimental group and the same volume of normal saline was injected in the control.The mice of both groups were let out the blood till death after 8, 16,24 or 36 hours separately.The mean titre of serum was 100 u/ml in 8 hours group and reached the peak（160 u/ml）in 16 hours group.The titre dropped in 24 hours group and would not be able to check out in the control.The induced interferon in 16 hours group that was treated with pancreatin for one hour under the condition of pH=2.0 and 56℃,which indicated that it might be a-interferon.

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