热毒清抗内毒素所致溶酶体和线粒体损伤的实验研究

作者	单位
李鸣真	同济医科大学中西医结合研究所
叶望云	同济医科大学中西医结合研究所
皇甫永穆	同济医科大学医学分子生物学研究室
邓益明	同济医科大学中西医结合研究所
陆付耳	同济医科大学中西医结合研究所
梁驹卿	同济医科大学医学分子生物学研究室

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中文摘要:

本实验制作内毒素所致家兔 DIC 模型,观察到肝细胞溶酶体和线粒体在超微结构与功能方面均受到明显损害。而应用热毒清(原名"抗炎6号")注射液之模型的动物肝细胞超微结构基本正常,溶酶体标志酶酸性磷酸酶(ACP)明显减低;线粒体呼吸功能、ATP 酶、过氧化脂质接近正常,与模型组比较差异非常显著。体外实验结果一致。提示热毒清有拮抗内毒素所致溶酶体和线粒体损伤的功能。

英文摘要:

A rabbit endotoxic DIC model was preliminarily performed.The structures of lysosomes and mitochondriae in liver cells were evidently destroyed as observed under electronic microscope, whereas those rabbits pretreated withRe Du Qing(RDQ,formerly named anti-inflammatory No.6) -a mixture of Chinese traditional herbs providing antipyretic and detoxifying action,ahowed prinipally normal ultrastructure in liver cells.In lysosomal functional studies,the activity of the lysosomal marker enzyme-acid phosphate(ACP)was 79.0±4.7% (M±SD)in model group,higher than pretreated group(54.01±4.0%,P<0.01).Studies on the mitochondrial function showed that the significant criteria of the respiratory activity of mitochondria-respiratory control ratios(RCR)was 2.83±1.08 in model group and markedly lower than pretreated group(5.46±1.25,P<0.01).Mitochon- drial ATPase activity(μ mol pi/min/mg pr.)was lower in model group(0.280±0.015)than in pretreated group(0.341±0.018, P<0.05).Lipid peroxide(LPO)in liver homogenates and serum were 1.86±0.43 n tool MDA/mg pr.and 12.26±0.84 n mol MDA/ml respectively in model group,whereas in pretreated group they gave a much lower value(1.19±0.12 and 6.55±2.97)respectively.Those data showed very significant difference between two groups(P<0.01).All the above indices of pretreated group yielded values close to those of normal control group.The results of experimental study in vitro were identical to those of experimental study in vivo.These experimental studies suggested that RDQ provide antagonistic effect on endotoxin induced damage of lysosomes and mitochondriae.

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