复方中药注射液对人癌细胞体外杀伤效应及其对细胞周期的影响

作者	单位
张永健	河北医学院北京市肿瘤防治研究所药理组
王耐勤	河北医学院北京市肿瘤防治研究所药理组
许树旭	河北医学院北京市肿瘤防治研究所药理组
刘彤	河北医学院北京市肿瘤防治研究所药理组

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中文摘要:

复方中药注射液（ICHM）对人胃癌细胞 BGC-823和人乳癌细胞 MCF-7均有较强的杀伤作用。IC₅₀分别为0.02g 生药/mI 和0.018g生药/ml。流式细胞分析仪测定表明,ICHM 使 BDC-823细胞停止在 G₁期,导致 S 期细胞显著减少。通过³H-TdR 掺入试验观察到,ICHM 能抑制 BGC-823细胞的 DNA 合成。

英文摘要:

Previous studies had shown that injection of Chinese herbal mixture（ICHM）had antitumor and anti-metastatic actions on various transplantable animal tumors.In the present study,the authors observed the obvious killing actions of ICHM on human gastric cancer cell line BGC-823 and breast cancer cell line MCF-7 in vitro.The IC₅₀were 0.02 g crude drug/ml and 0.018 g crude drug/ml respectively.The study of the effect of ICHM on the cell-cycle of BGC-823 cell showed that ICHM decreased the number of cells in Sphase and increased the number of cells in G₁ phase significantly. The results indicated that the BGC-823 cells was blocked on S phase by ICHM.Meanwhile.ICHM reduced 3^H-TdR incorporation into DNA,the cpm value was much lower than that of the control.

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