肾主生殖的实验研究

作者	单位
俞瑾	妇产科医院妇产科医院
陈红英	妇产科医院妇产科医院
毛秋芝	妇产科医院妇产科医院
李超荆	妇产科医院妇产科医院
金丕奂	卫生系电子计算机室
刘昌茂	基础部病理教研室
施大文	药学系生药教研室

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中文摘要:

为建立多囊卵巢小鼠模型,分别给不同日龄(5,8,11,14,17,21日龄)昆明种小鼠注射1mg 睾酮,结果提示以5日龄以内的昆明种小鼠注射睾酮,制成排卵功能失调的动物模型最佳。在此基础上对2,5,8,11,14日龄昆明种小鼠进行睾酮处理,并将治疗月经失调病中常用补肾药分离成水溶和脂溶部分,给上述各组小鼠服药并作对照观察。结果2日龄组卵巢均为多囊卵巢;补肾药水溶部分使各日龄组子宫增重,使多囊卵巢内间质腺细胞肥大,使8日龄以上小鼠卵巢增重;补肾药脂溶部分则无以上作用。提示补肾药中水溶部分是调节卵巢功能的主要部分。

英文摘要:

To elucidate the mechanism of TCM in treating polycystic ovary syndrome,the authors tried to produce experimental PCO by sterilizing Kunming mice with 1 mg testosterone in various ages of day （5,8,11,14,17,21 days）.Every litter consisting of 10 young mice was divided into two groups: testosterone given group（T）and control group（C）.At the age of 72～75 day,ovaries and uteri were weighed and all ovaries were investigated histologically.Mating was attempted in the two groups. 19 of the 20 ovaries in T₅ group,but none of the other groups showed polycystic features.4 of the 15 mice in T₅ group and all of the other groups resulted in pregnancy（P<0.05）.It was suggested that polycystic ovary was formed in the Kunming mice at age of less than 5 days administered with testosterone. For observation of tonifying Kidney drugs on the ovaries and uteri of the polycystic ovary model in Kunming.mice,testosterone was given to Kunming mice of various age（2,5,8,11,14 days）.The tonifying Kidney drugs for anovulatory menstrual disorder were separated into two parts:water soluble part（MS）and ester soluble part（ML）.At the age of 56 days（8 weeks）,two parts of the drug were given for 14 days to every group of the mice respectively.One day after the administration, uteri were weighed,ovaries were investigated microscopically,and mating was attempted in both groups.All uteri in mice taken water soluble drugs（MS）increased in weight（P<0.05）,but not in the ML groups.All ovaries in groups of 2-day age（T₂）and most ovaries in T₅ groups showed polycystic features.No significant difference of the ovulatory and pregnant rates was found between all MS groups and ML groups with their control groups（CS,CL）respectively.Interstitial glands in the ovary of the MS groups were increased in size,but riot the ML groups.It was suggested that the MS of tonifying Kidney drugs had an important effect on the dysfunctional ovary in mice,and this polycystic ovary animal model may be taken as the experimental animal model in TCM study on regulating reproductive endocrinotogical function.

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