氧化苦参碱延长小鼠异体移植心肌存活期及其免疫机理的研究

作者	单位
秦泽莲	第三医院成形研究中心
庄红明	第三医院成形研究中心
刘燕	第三医院成形研究中心
邓鸿业	北京医科大学免疫学教研室
邓玉兰	北京医科大学免疫学教研室
边建朝	北京医科大学免疫学教研室
王宝勤	北京医科大学免疫学教研室

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中文摘要:

本实验观察到肌肉注射氧化苦参碱具有明显延长小鼠异体游离移植心肌存活期的作用,并随剂量加大其作用增强。检查药物对移植和非移植小鼠的脾脏淋巴细胞转化和免疫球蛋白分泌细胞的影响。实验发现,移植术后10天小鼠体外脾脏细胞自发增殖增强,对刀豆蛋白 A(ConA)刺激的转化反应则明显减弱。氧化苦参碱显著抑制正常小鼠的脾细胞的自发增殖及对 ConA 或脂多糖(LPS)刺激的转化反应,而对手术已使之显著降低的脾细胞对 ConA 刺激的转化反应无影响。这种选择性抑制作用可能利于避免用其他常规非特异免疫抑制剂所带来的危害。

英文摘要:

Oxymatrine is an extract from Sophora flavescens Ait,A daily dose of 75 mg/kg or 225 mg/kg of oxymatrine was given to the recipient intramuscularly for 14 days.The survival time of cardiac tissue allograft was prolonged significantly to 12.2 days (at the dose of 75 mg/kg,P<0.05) and 15. 7 days (at the dose of 225 mg/kg,P<0.001) by oxymatrine,while that in the control group was 10. 8 days. The effects of oxymatrine on immune.function in BABL/c mice with or without heart allograft were further studied.Experiments showed that in vitro spontaneous proliferation of spleen cells increased markedly on the 10th day after transplantation,while the proliferation response to Con A of spleen cells decreased.The spontaneous proliferation and proliferation responses to Con A or to LPS of spleen cells could be inhibited significantly in normal mice by oxymatrine.The proliferation response to LPS of spleen cells and RPFC was inhibited obviously in transplanted mice by oxymatrine,However,oxymatrine did not affect the proliferation response to Con A of spleen cells, which had been decreased after transplantation.The results suggested that this drug exhibited selective immtmo-suppression on function of B cells without obvious effect on T cell function in transplanted mice.This characteristics of the drug seemed beneficial for avoiding aide-effect produced by the conventional immuno-suppressive agents.

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