| 陈名道,邝安堃,陈家伦,蔡厚明,李凤英,唐金凤,陈梦月.助阳药对可的松大鼠模型下丘脑单胺类神经递质作用的研究[J].中国中西医结合杂志,1990,(5):292-294,261 |
| 助阳药对可的松大鼠模型下丘脑单胺类神经递质作用的研究 |
| Study on Effect of Monoamine Neurotransmitters in Hypothalamus of Cortisol Rats Model with Yang(阳)-Strenthening Herbs |
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| DOI： |
| 中文关键词: 助阳药 可的松大鼠模型 单胺突神经递质 高效液相色谱——电化学检测 |
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| 中文摘要: |
| 用高效液相色谱——电化学检测联用,以樟脑磺酸为离子对试剂,测定可的松阳虚大鼠及正常大鼠下丘脑单胺类神经递质,并观察用助阳药附子、肉桂、肉苁蓉、仙灵脾后的效果。发现:可的松阳虚大鼠下丘脑去甲肾上腺素(NA)较正常大鼠下降,肾上腺素(A)升高(P 均<0.05),用助阳药后能恢复正常。助阳药使可的松阳虚大鼠及正常大鼠多巴胺(DA)均升高(P 分别<0.05及 P<0.01),3.4-二羟基苯乙酸(DOPAC)下降(P<0.001),并使正常大鼠5-羟色胺(5-HT)升高(P<0.001),用药后阳虚及正常大鼠都表现为 DA/DOPAC 及5-HT/5-羟吲哚醋酸(5-HIAA)比值升高(P<0.01～0.001)的共同特点,提示助阳药似有抑制下丘脑单胺氧化酶活性的作用 |
| 英文摘要: |
| Eight monoamine neurotransmitters in hypothalamus of normal and cortisol-induced Yang- deficiency rats were measured with HPLC-ECD.These neurotransmitters included:(1)Nor- adrenaline(NA);(2)Adrenaline(A);(3)3-methoxy-4-hydroxyphenyl glycol(MHPG);(4)3,4- dihydroxyphenylacetic acid(DOPAC);(5)Dopamine(DA);(6)5-hydroxyindole-3-acetic acid(5- HIAA);(7)Homovanillic acid(HVA);and(8)Serotonin(5-HT).With the mobile phase of 10% methanol-0.15M chloroacetic acid-sodium hydroxide buffer,the neurotransmitters might be well separated,the sensitivity was similar to that of gas chromatography-mass spectrogram with the lower limit of 0.1ng,this assay was simple and might be accomplished with half an hour. The Yang-strengthening Chinese herbs Radix Aconiti Praeparata,Cortex,Cinamomi, Epimedium brevicorum and Herba Cistanchis were used in the study and the following were founded: (1)In the hypothalamus of cortisol-induced Yang-deficiency rat NA decreased and A increased when compared with that of the normal controls(both P<0.05).(2)After 4 weeks of administration of Yang-strenthening herbs,the NA and A of the Yang-deficiency rats returned to nearly normal and it suggested these herbs have the effect of"supporting the right and recovering the source";DA markedly increased both in control rats and Yang-deficiency rats(P<0.05,P<0.001 respectively); DOPAC obviously decreased in both groups(both P<0.001);5-HT and 5-HIAA increased in the control rats(both P<0.001)and HVA significantly decreased in Yang-deficiency rats.(3)The ratio of DA/DOPAC and 5-HT/5-HIAA markedly increased both in the control and Yang-deficiency rats after using Yang-strengthening herbs(P<0.01～0.001)and it suggested Yang-strengthening herbs could inhibit the activity of monoamine oxidase in hypothalamus. |
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