冬虫夏草、大黄及肾大部切除大鼠血清对肾小管上皮细胞生长的影响

作者	单位
田劲	解放军总医院肾脏内科北京 100853
陈香美	解放军总医院肾脏内科北京 100853
黎磊石	解放军总医院肾脏内科北京 100853

摘要点击次数: 1520

全文下载次数: 809

中文摘要:

本研究利用体外肾小管上皮细胞培养及氚标记的胸腺嘧啶核普(3~H-TdR)掺入技术,观察了冬虫夏草(虫草)对细胞生长的影响,结果表明虫草可明显促进3~H-TdR 向肾小管上皮细胞内掺入(虫草组11060.63±603.04CPM,对照组8323.83±836.72CPM,P<0.001)。为此考虑虫草减轻肾小管损伤的作用之一在于促进细胞的修复。此外,我们还观察了大鼠5/6肾切除后血清及中药大黄对肾小管上皮细胞的影响,结果显示5/6肾切除后血清能明显刺激3~H-TdR 掺入到上皮细胞内,而大黄则可抑制肾小管上皮细胞的生长。

英文摘要:

Primary cultured rat tubular epithelium was utilized to investigate the effect of Cordyceps senensis (CS) on cellular proliferation and metabolism.Judging from incorporation rate of 3~H-TdR, it was.found that the addition of serum containing CS metabo]ites into the culture media could promote the DNA synthesis of tubular ceils profoundly (P<0.001).In association with its beneficial effects on gentamycin nephrotoxity in vivo study,it is indicated that CS could enhance the regeneration of injured tubular cells.In addition,sera obtained from 5/6 nephrectomized rats (5/6 NT) and rhubarb treated rats were studied to see their effects on tubular cells growth.The results showedthat the serum of 5/6NT rats could promote the DNA synthesis of tubular epithelium, while the presence of experimental serum of rhubarb in culture median markedly inhibited the DNA synthesis of cells.

关闭