丹参注射液对鸡胚额骨分离细胞培养生长影响的组织化学观察

作者	单位
徐荣辉	上海市伤骨科研究所 (上海200025)
柴本甫	上海市伤骨科研究所 (上海200025)
朱雅萍	上海市伤骨科研究所 (上海200025)

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中文摘要:

孵育12天的鸡胚额骨用胰酶制成分离细胞悬液。共获得3×10⁶个细胞,均分为5份,分别加 Eagle 培养液或0.1%、0.2%、0.4%和0.8%丹参 Eagle 培养液,在CO₂培养箱内进行培养。逐日进行活体观察。至培养26天收获。标本作 HE、AS、ARS 染色和 AlP-AcP 反应以进行比较。发现:丹参能促进成骨细胞样细胞成熟,分泌胶原性物质和 AlP,并使钙盐在胶原基质上沉积,形成骨小结节。但是过高浓度的丹参能导致对成骨细胞样细胞生长的抑制。丹参的合适实验浓度为0.2%。

英文摘要:

12-day embryonic chicken frontal bone digested with trypsin to prepare the suspension of isolated bone cells.3×10⁶ cells were harvested altogether.The cells were divided equally into five parts.Then the Eagle medium and 0.1%,0.2%,0.4% and 0.8% Radix Salviae Miltiorrhizae in Eagle medium were added respectively and cultured in 5% CO₂ incubator.It was observed under the inverted microscope every day.At the 26th day of culture,the cells were studied.The specimens were stained with H.E.,Alcian Blue-Sirius Red, Alizarin Red S staining and alkaline phosphatase-acid phosphatase reaction for comparison.It Was found that the maturation of the osteoblast-like cells could be accelerated by Radix Salviae Miltiorrhizae.Secretion of the collagenous substance,positive alkaline phosphatase reaction and deposition of mineral on the collagenous substance,forming bone nodules were found to be enhanced.But unduly high concentration of Radix Salviae Miltiorrhizae could lead to inhibition of osteoblast-like cell growth.The optimal concentration of Radix Salviae Miltiorrhizae was 0.2% in culture medium.

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